FAQ-967
Do you have a protocol for manual purification of 6xHis-tagged proteins expressed in E. coli using Ni-NTA Superflow?
Yes, please follow either of the QIAGEN Supplementary Protocols:
Related products
Ni-NTA Superflow 96 BioRobot Kit
The Ni-NTA Superflow 96 BioRobot Kit provides automated purification of up to 300 µg recombinant protein from 96 samples in parallel (standard protocol, 5 ml culture volume per sample). Cleared bacterial cell lysates flow onto a filter plate preloaded with Ni-NTA Superflow by the BioRobot workstation. This unique 96-well metal-chelate affinity-chromatography module strongly and selectively binds His-tagged proteins. Ready-to-run protocols are provided for purification under native or denaturing conditions.
Ni-NTA Superflow
Ni-NTA Superflow is an affinity chromatography matrix for purifying recombinant proteins carrying a His tag.
Histidine residues in the His tag bind to the vacant positions in the coordination sphere of the immobilized nickel ions with high specificity and affinity. Cleared cell lysates are loaded onto the matrices. His-tagged proteins are bound, and other proteins pass through the matrix. After washing, His-tagged proteins are eluted in buffer under native or denaturing conditions.