FAQ-45
What are the expected DNA yields from different tissues using the QIAamp DNA Mini Kit?
The expected DNA yields from different tissues using the QIAamp DNA Mini Kit are as follows:
| Sample | Nucleic acid yield without RNase A treatment (ug) | DNA yield with RNase A treatment (ug) |
| Blood (200 ul) | 4–12 | 4–12 |
| Buffy coat (200 ul) | 25–50 | 25–50 |
|
Cultured cells (5 x 10e6) |
20–30 | 15–20 |
| Liver (25 mg) | 60–115 | 10–30 |
| Brain (25 mg) | 35–60 | 15–30 |
| Lung (25 mg) | 8–20 | 5–10 |
| Heart (25 mg) | 25–45 | 5–10 |
| Kidney (25 mg) | 40–85 | 15–30 |
| Spleen (10 mg) | 25–45 | 5–30 |
DNA was purified with QIAamp Kits following standard protocols.
Related products
QIAamp DNA Kits
QIAamp DNA Kits provide silica-membrane-based nucleic acid purification from tissues, swabs, CSF, blood, body fluids or washed cells from urine. In addition, genomic and mitochondrial DNA can be purified from small amounts of fresh or frozen blood, tissue and dried blood spots. Mechanical homogenization is not required as the tissues are lysed enzymatically. DNA purification from 1–12 samples can be automated on the QIAcube Connect using the dedicated QIAamp DNA Mini QIAcube Kit. Purification of DNA using the QIAamp DNA Micro Kit and QIAamp DNA Mini Kit is also automatable on the QIAcube Connect.
QIAamp DNA Mini standard protocols can also be executed using the TRACKMAN Connected system.