RNase A

• Selectively cleaves single-stranded RNA
• Active under a wide range of reaction conditions
• DNase-free and quality-controlled
• Does not need to be boiled before use 

RNase A is an endoribonuclease that breaks down single-stranded RNA molecules by hydrolysis 3' of pyrimidine residues (cytosine and uracil). RNase A is a basic protein of 124 amino acids, with a molecular weight of approximately 13.7 kDa. RNase A catalyzes the transphosphorylation and degradation of RNA by cleaving specifically at several sites on a single stranded RNA polynucleotide chain at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds. Ribonucleases like RNase A do not hydrolyze DNA because the DNA lacks 2′-OH groups essential for forming cyclic intermediates. RNase A is widely used to remove contaminating RNA during the isolation of plasmid and genomic DNA.  

DNase-free Ribonuclease A is quality-controlled and ready to use for all applications where digestion of RNA is required.

QIAGEN Ribonuclease A is DNase-free and quality-controlled for use in plasmid purification procedures for the digestion of RNA.  

RNase A is widely used to remove contaminating RNA during the isolation of plasmid and genomic DNA. RNase A is also used to remove contaminating RNA from DNA samples after extraction or PCR, especially if downstream applications (e.g., cloning or sequencing) require RNA-free DNA.  

RNase A is very active under a wide range of reaction conditions and is difficult to inactivate. 

QIAGEN RNase A does not need to be boiled before use. 

Before use in plasmid and DNA purification, centrifuge QIAGEN RNase A briefly, and then add into Buffer P1 to obtain a final concentration of 100 μg/mL. Store Buffer P1 at 2–8°C after addition of RNase A. For detailed instructions on RNase A usage during plasmid and DNA purification, please refer to the corresponding kit handbook.  

• Plasmid and genomic DNA preparation
• Removal of RNA contamination from recombinant protein preparations
• Cleanup of RNA from enzymatic reactions