The updated QIAGEN EndoFree Plasmid Kit delivers faster, more sustainable plasmid DNA preparation for transfection by replacing Triton X-100 with a REACH-compliant detergent and shortening a key incubation from 30 minutes on ice to 5 minutes at room temperature. The kit now achieves extremely low endotoxin levels (as low as 0.001 EU/µg DNA, 2–40x below vaccine-relevant limits), including for challenging low-copy plasmids. Validation in robust HeLa cells, endotoxin-sensitive CHO cells and primary human CD3+ T cells shows high transfection efficiencies with no adverse impact on cell morphology. This makes the updated kit well-suited for demanding transfection workflows, particularly in sensitive or primary cell systems, while supporting lab sustainability and efficiency goals.
Thuvaraha Kalasegaran, Uritza von Groll, Sara Khani, Peter Porschewski and Yvonne Ponath
QIAGEN GmbH, Hilden, Germany