QIAcuity Digital PCR System

Pour les applications de PCR digitale sur nanoplaque

S_9648_dPCR_instruments_QIAcuity
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QIAcuity One, 2plex Device

Cat. No. / ID:  911001

Instrument de PCR digitale à une seule plaque permettant la détection de jusqu’à 2 colorants fluorescents, rouleau, clé USB et QIAcuity Software Suite : comprend 1 visite de maintenance préventive. Une garantie de 1 an couvrant pièces, main-d’œuvre et déplacement est également incluse.
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Format
One
Four
Eight
Multi-channel detection
2plex
5plex
Service Options
Device
System
System FUL-2
Le QIAcuity est destiné aux applications de biologie moléculaire. Ce produit n’est pas conçu pour le diagnostic, la prévention ou le traitement des maladies. Par conséquent, les caractéristiques de performances du produit dans un contexte clinique (diagnostic, pronostic, thérapie ou banque de sang) sont inconnues.
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Features

  • Système entièrement intégré
  • Format évolutif (instruments à 1, 4 et 8 plaques)
  • Capacités de multiplexage avancées (jusqu’à 5-plex)
  • Cadence d’analyse flexible
  • Résultats complets en 2 heures environ

Product Details

Le QIAcuity Digital PCR System est conçu pour produire des résultats précis de quantification multiplex pour la détection des mutations, les variations du nombre de copies (Copy Number Variation, CNV), les études d’expression génique, l’analyse d’édition génique, et bien plus encore. Ce système à base de nanoplaques intègre une méthode de dPCR classique de partitionnement, thermocyclage et imagerie dans une plateforme automatisée et autonome, réduisant au minimum le temps d’intervention.

Ce système s’utilise en conjonction avec les nanoplaques et accessoires QIAcuity.

Explorez la démonstration virtuelle pour en savoir plus sur QIAcuity.

Performance

Le QIAcuity Digital PCR System rend la quantification absolue accessible et abordable pour tous les laboratoires. Permettant un fonctionnement autonome, l’automatisation intègre et simplifie toute la méthode de PCR digitale de partitionnement, thermocyclage et imagerie dans un seul instrument avec un temps d’intervention minimal. Il est également facile d’adapter vos dosages de qPCR actuels au QIAcuity Digital PCR System. La manipulation des plaques ne requiert aucune modification par rapport à la qPCR. La configuration des dosages est donc rapide et les résultats sont obtenus en 2 heures environ.

Caractéristiques et spécifications – des instruments QIAcuity

Caractéristique QIAcuity One QIAcuity Four QIAcuity Eight
Plaques traitées 1 4 8
Canaux de détection (multiplexage) 2 ou 5 5 5
Thermocycleur(s) 1 1 2
Délai d’obtention du résultat Environ 2 h

Première plaque en 2 h environ

Une plaque supplémentaire toutes les 60 min env.

Première plaque en 2 h environ

Une plaque supplémentaire toutes les 30 min env.

Débit (échantillons traités au cours d’une journée)

Jusqu’à 384 (96 puits)

Jusqu’à 96 (24 puits)

Jusqu’à 672 (96 puits)

Jusqu’à 168 (24 puits)

Jusqu’à 1248 (96 puits)

Jusqu’à 312 (24 puits)

Principle

Vous pouvez obtenir, en seulement 3 étapes simples, le résultat de dPCR que vous voulez en 2 heures environ : pipettez et chargez, effectuez l’expérience, puis analysez les résultats.

Vous trouverez ici une description du principe de la réaction de dPCR sur nanoplaque.

Procedure

Comme dans les expériences de qPCR, la préparation des échantillons comprend le transfert du mélange principal, des sondes et des amorces sur une nanoplaque de 96 ou 24 puits, suivi de l’addition des échantillons. Le système intègre le partitionnement, le thermocyclage et l’imagerie dans un seul instrument entièrement automatisé qui permet aux utilisateurs de passer de l’échantillon aux résultats en moins de 2 heures. Il est possible d’effectuer l’analyse à l’aide de la suite logicielle, qui donne la concentration de la séquence cible en copies par microlitre et permet le contrôle qualité pour les échantillons positifs ou NTC. Cette analyse peut également être effectuée sur des ordinateurs distants au sein du même réseau local (Local Area Network, LAN).

Applications

Associés avec les QIAcuity Nanoplaques et les kits de PCR QIAcuity, les instruments QIAcuity facilitent les applications de PCR digitale telles que :

  • La détection des mutations rares
  • L’analyse des variations du nombre de copies
  • L’analyse de l’expression génique
  • La détection des agents pathogènes
  • Le génotypage
  • La recherche sur les miARN

Software

La QIAcuity Software Suite fournie avec l’instrument et installée sur un ordinateur distinct contrôle un ou plusieurs instruments QIAcuity, soit via une connexion directe, soit via un réseau local (Local Area Network, LAN) existant. À l’aide de la QIAcuity Software Suite, les expériences, échantillons et mélanges principaux de PCR digitale peuvent être définis, attribués à des nanoplaques et transférés sur l’instrument QIAcuity. Après traitement, il est possible d’analyser les données, de créer des rapports et d’exporter les données pour une analyse externe. Le logiciel offre de multiples fonctionnalités de modèles pour faciliter la réutilisation de plans de plaque ou de paramètres d’analyse de plaques, transformant encore davantage votre pratique de la PCR digitale.

Lorsqu’il est intégré à un réseau local, l’ordinateur héberge la fonction QIAcuity Software Suite en tant que serveur accessible via LAN aux ordinateurs clients. Cela permet à plusieurs utilisateurs d’accéder au logiciel depuis d’autres pièces ou bureaux et d’analyser les données dans un navigateur classique, sans devoir installer le logiciel sur plusieurs ordinateurs ni utiliser de connexions internet pour accéder aux données et pour les transmettre.

Services

Protégez votre instrument grâce aux diverses solutions de service QIAGEN. Renseignez-vous sur un contrat de service spécifique pour répondre à vos besoins.

Supporting data and figures

Service Plans

QIAcuity One 2plex Full Agreement

Cat. No. / ID:  9245362

Une réparation d’instrument QIAcuity One 2plex sur site, comprenant déplacement, pièces et main-d’œuvre pendant un an. Délai de réponse de deux jours ouvrables. Comprend une visite de maintenance préventive pendant la période couverte par le contrat complet.
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QIAcuity One Preventive Subscription

Cat. No. / ID:  9245355

Une visite de maintenance préventive (MP) sur site, comprenant déplacement, main-d’œuvre et pièces de MP. Comprend une remise de 10 % sur les prestations de réparation pendant la période d’abonnement de prévention, ainsi que l’établissement d’un compte rendu de visite conforme aux BPF/BPL.
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QIAcuity Four Preventive Subscription

Cat. No. / ID:  9245356

Une visite de maintenance préventive (MP) sur site, comprenant déplacement, main-d’œuvre et pièces de MP. Comprend une remise de 10 % sur les prestations de réparation pendant la période d’abonnement de prévention, ainsi que l’établissement d’un compte rendu de visite conforme aux BPF/BPL.
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QIAcuity Eight Preventive Subscription

Cat. No. / ID:  9245357

Une visite de maintenance préventive (MP) sur site, comprenant déplacement, main-d’œuvre et pièces de MP. Comprend une remise de 10 % sur les prestations de réparation pendant la période d’abonnement de prévention, ainsi que l’établissement d’un compte rendu de visite conforme aux BPF/BPL.
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QIAcuity One 2plex Core Agreement

Cat. No. / ID:  9245358

Une prestation de réparation sur site et une visite de maintenance préventive sur site, comprenant déplacement, pièces et main-d’œuvre pendant un an. Délai de réponse de cinq jours ouvrables. Comprend une remise de 10 % sur une prestation de réparation supplémentaire en cours de période contractuelle.
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QIAcuity One 5plex Core Agreement

Cat. No. / ID:  9245359

Une prestation de réparation sur site et une visite de maintenance préventive sur site, comprenant déplacement, pièces et main-d’œuvre pendant un an. Délai de réponse de cinq jours ouvrables. Comprend une remise de 10 % sur une prestation de réparation supplémentaire en cours de période contractuelle.
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QIAcuity Four Core Agreement

Cat. No. / ID:  9245360

Une prestation de réparation sur site et une visite de maintenance préventive sur site, comprenant déplacement, pièces et main-d’œuvre pendant un an. Délai de réponse de cinq jours ouvrables. Comprend une remise de 10 % sur une prestation de réparation supplémentaire en cours de période contractuelle.
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QIAcuity Eight Core Agreement

Cat. No. / ID:  9245361

Une prestation de réparation sur site et une visite de maintenance préventive sur site, comprenant déplacement, pièces et main-d’œuvre pendant un an. Délai de réponse de cinq jours ouvrables. Comprend une remise de 10 % sur une prestation de réparation supplémentaire en cours de période contractuelle.
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QIAcuity One 2plex Basic Agreement

Cat. No. / ID:  9245401

Une réparation d’instrument QIAcuity One 2plex sur site, comprenant déplacement, pièces et main-d’œuvre pendant un an. Délai de réponse de cinq jours ouvrables. Comprend une visite de maintenance préventive ou une prestation de contrôle pendant la période couverte par le contrat de base.
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Contrat de base pour QIAcuity One 5plex

Cat. No. / ID:  9245402

Une réparation d’instrument QIAcuity One 5plex sur site, comprenant déplacement, pièces et main-d’œuvre pendant un an. Délai de réponse de cinq jours ouvrables. Comprend une visite de maintenance préventive ou une prestation de contrôle pendant la période couverte par le contrat de base.
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QIAcuity One 5plex Full Agreement

Cat. No. / ID:  9245363

Une réparation d’instrument QIAcuity One 5plex sur site, comprenant déplacement, pièces et main-d’œuvre pendant un an. Délai de réponse de deux jours ouvrables. Comprend une visite de maintenance préventive pendant la période couverte par le contrat complet.
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QIAcuity Four Full Agreement

Cat. No. / ID:  9245364

Une réparation d’instrument QIAcuity Four sur site, comprenant déplacement, pièces et main-d’œuvre pendant un an. Délai de réponse de deux jours ouvrables. Comprend une visite de maintenance préventive pendant la période couverte par le contrat complet.
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QIAcuity Eight Full Agreement

Cat. No. / ID:  9245365

Une réparation d’instrument QIAcuity Eight sur site, comprenant déplacement, pièces et main-d’œuvre pendant un an. Délai de réponse de deux jours ouvrables. Comprend une visite de maintenance préventive pendant la période couverte par le contrat complet.
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QIAcuity One Installation & Training

Cat. No. / ID:  9245352

Installation du matériel de l’instrument et du logiciel système. Comprend une formation pratique aux applications, couvrant l’utilisation de l’instrument et la qualification des membres du laboratoire. La main-d’œuvre, le déplacement et le kit d’installation (le cas échéant) sont inclus.
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QIAcuity Four Installation & Training

Cat. No. / ID:  9245353

Installation du matériel de l’instrument et du logiciel système. Comprend une formation pratique aux applications, couvrant l’utilisation de l’instrument et la qualification des membres du laboratoire. La main-d’œuvre, le déplacement et le kit d’installation (le cas échéant) sont inclus.
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QIAcuity Eight Installation & Training

Cat. No. / ID:  9245354

Ce service associe l’installation avec la formation aux applications, permettant ainsi aux clients de se familiariser avec le matériel, les logiciels et les applications exécutées sur les plateformes QIAGEN, et accélérant la qualification professionnelle des membres du laboratoire.
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Resources

Instrument User Manuals (16)
Network connection of QIAcuity instruments
For QIAcuity Software Suite 1.2: Updating VPF for archived or exported plates
For QIAcuity User Manual – Updated QIAcuity Nanoplate Partition Volumes
Updating QIAcuity Software Suite 1.1.3 and Control Software 0.5.2.18 to QIAcuity Software 2.0
For use with QIAcuity systems

July 2021
User manual for QIAcuity instruments and QIAcuity Software 1.2
User manual for QIAcuity instruments and QIAcuity Software 2.0
User manual for QIAcuity instruments and QIAcuity Software 2.1
User manual for QIAcuity instruments and QIAcuity Software 2.1.8
User manual for QIAcuity instruments and QIAcuity Software 2.2
User manual for QIAcuity instruments and QIAcuity Software 2.5
QIAcuity backup and restore scripts for QIAcuity Software Suite versions 2.0, 2.1.7, 2.1.8, and 2.2


QIAcuity backup and restore scripts for QIAcuity Software Suite version 2.5 or higher

Latest Software Version (9)

Version 2.5.0.1

 

The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. This software is also used for the configuration of the system and provides access to the QIAcuity user management. The QIAcuity Software Suite is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments.  For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).

The new version 2.5 of the QIAcuity Software Suite supports assay development by providing an essential temperature gradient functionality. It also offers a new feature that provides calculation of initial concentration of samples by using various dilution factors. In addition, concentration units may be converted into various pre-defined or user-defined units. The new software also offers an integrity value and concentration value per group for up to 5-plex added to the multiple occupancy CSV file export, for example, for the evaluation of AAV (adeno-associated virus) assays and for drop-off assays.

In this new version the initial loading time and the time for recalculation of 1D/2D scatterplot and for signal map image has been reduced, leading to a much faster performance.

Detailed information about the QIAcuity Software Suite version 2.5 is available in the Release Note, which can also be downloaded under section “Software Release Notes”.

 

Note: The latest Software Suite version 2.5 is only compatible with the latest Control Software (CSW) version 2.5. If only one software component is updated, no connection between the Software Suite and the CSW can be established.

Important: Please follow the instructions provided in the QIAcuity User Manual for software version 2.5 and in the Release Note.

It is strongly recommended to update the QIAcuity Software Suite first before proceeding with the QIAcuity Control Software!

Please contact QIAGEN Technical Services if you are unsure and require technical support.

SHA1 checksum: D1690226299A75E077FA37C420A970FC71D56CBF

 

 

Version 7.0

 The Volume Precision Factor (VPF) offers a unique feature to secure precision of concentration results obtained from a QIAcuity dPCR run. 

In general, Nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. Potential variation of partition sizes in Nanoplate batches, caused by different microstructure molding forms, can be addressed by applying the batch specific VPF. Furthermore, the VPF includes well-specific volume information and therefore further increases precision of concentration calculation in each well of the Nanoplates.

 

Important note:

The Volume Precision Factor file version 7.0 is compatible with QIAcuity Software Suite version 2.1.8.23 or higher. All lower versions, namely 1.2.18, 2.0.20, 2.1.7.182, and 2.1.8.20, require a QIAcuity Software Suite patch to be installed prior the upload of VPF version 7.0. Please read the Release Note for QIAcuity Software Suite Volume Precision Factor (VPF) Patches for more information.

 If you are using QIAcuity Software Suite versions older than 2.1.8.23, please consider updating your Software Suite and the Instrument Control Software (CSW) to the most recent version prior the upload of VPF version 7.0. After updating to the latest QIAcuity Software Suite software version, no patching is needed. If you are not able to update your QIAcuity Software Suite, please run the patch for your Software Suite version following the instructions provided in the Release Note for QIAcuity Software Suite Volume Precision Factor (VPF) Patches.

 After downloading and updating the VPF file within the QIAcuity Software Suite, the VPF is applied automatically to the analysis of a corresponding Nanoplate batch. The VPF file includes information from all available microstructure molding forms and connected Nanoplate batches. It will be stored on the PC where the QIAcuity Software Suite is installed.



The following QIAcuity Software Suite Volume Precision Factor (VPF) patches have been released to enable compatibility with VPF file version 6.0 or higher for QIAcuity Software Suite version 1.2.18. As the abovementioned QIAcuity Software Suite versions do not allow loading a VPF zip file including more than 20 different individual VPF files, a patch is needed to allow the software for loading VPF zip files version 6.0 or higher. This technical limitation is solved from QIAcuity Software Suite version 2.1.8.23 onwards.

 If you are using the QIAcuity Software Suite version 1.2.18, please consider updating your Software Suite and the Instrument Control Software (CSW) to the most recent version. After updating to the latest software version, no patching is needed.

 If you are not able to update your QIAcuity Software Suite, please install the QIAcuity Software Suite VPF patch for QIAcuity Software Suite version 1.2.18 before loading VPF file version 6 or higher. Additional information and instructions may be found on the Release Note: QIAcuity Software Suite Volume Precision Factor (VPF) Patches.

The following QIAcuity Software Suite Volume Precision Factor (VPF) patches have been released to enable compatibility with VPF file version 6.0 or higher for QIAcuity Software Suite versions 2.0.20. As the abovementioned QIAcuity Software Suite versions do not allow loading a VPF zip file including more than 20 different individual VPF files, a patch is needed to allow the software for loading VPF zip files version 6.0 or higher. This technical limitation is solved from QIAcuity Software Suite version 2.1.8.23 onwards.

 If you are using the QIAcuity Software Suite version 2.0.20, please consider updating your Software Suite and the Instrument Control Software (CSW) to the most recent version. After updating to the latest software version, no patching is needed.

 If you are not able to update your QIAcuity Software Suite, please install the QIAcuity Software Suite VPF patch for QIAcuity Software Suite version 2.0.20 before loading VPF file version 6 or higher. Additional information and instructions may be found on the Release Note: QIAcuity Software Suite Volume Precision Factor (VPF) Patches.

The following QIAcuity Software Suite Volume Precision Factor (VPF) patches have been released to enable compatibility with VPF file version 6.0 or higher for QIAcuity Software Suite version 2.1.7.182. As the abovementioned QIAcuity Software Suite versions do not allow loading a VPF zip file including more than 20 different individual VPF files, a patch is needed to allow the software for loading VPF zip files version 6.0 or higher. This technical limitation is solved from QIAcuity Software Suite version 2.1.8.23 onwards.

 If you are using the QIAcuity Software Suite version 2.1.7.182, please consider updating your Software Suite and the Instrument Control Software (CSW) to the most recent version. After updating to the latest software version, no patching is needed.

 If you are not able to update your QIAcuity Software Suite, please install the QIAcuity Software Suite VPF patch for QIAcuity Software Suite version 2.1.7.182 before loading VPF file version 6 or higher. Additional information and instructions may be found on the Release Note: QIAcuity Software Suite Volume Precision Factor (VPF) Patches.

The following QIAcuity Software Suite Volume Precision Factor (VPF) patches have been released to enable compatibility with VPF file version 6.0 or higher for QIAcuity Software Suite version 2.1.8.20. As the abovementioned QIAcuity Software Suite versions do not allow loading a VPF zip file including more than 20 different individual VPF files, a patch is needed to allow the software for loading VPF zip files version 6.0 or higher. This technical limitation is solved from QIAcuity Software Suite version 2.1.8.23 onwards.

 If you are using the QIAcuity Software Suite version 2.1.8.20, please consider updating your Software Suite and the Instrument Control Software (CSW) to the most recent version. After updating to the latest software version, no patching is needed.

 If you are not able to update your QIAcuity Software Suite, please install the QIAcuity Software Suite VPF patch for QIAcuity Software Suite version 2.1.8.20 before loading VPF file version 6 or higher. Additional information and instructions may be found on the Release Note: QIAcuity Software Suite Volume Precision Factor (VPF) Patches.

The QIAcuity backup and restore scripts are a standalone solution to backup all relevant user data of the QIAGEN Software Suite for disaster recovery and restore the data on a new or existing QIAGEN Software Suite installation. The following QIAcuity Software Suite versions are supported: 2.0, 2.1.7, 2.1.8, and 2.2. The backup can be conducted manually or automated by using of the Windows task scheduler.

Note: Windows Admin permission is needed to setup and perform an automated backup and for manual backup and restore.

Please read the QIAcuity Software Suite Backup and Restore Scripts document for more information and instructions how to use the scripts.

 

The QIAcuity backup and restore scripts are a standalone solution to backup all relevant user data of the QIAGEN Software Suite for disaster recovery and to restore the data on a new or existing QIAGEN Software Suite installation. The backup supports the QIAcuity Software Suite version 2.5 or higher and can be conducted manually or automatically by using of the Windows Task Scheduler.

Note: Windows Admin permission is needed to setup and perform an automated backup and for manual backup and restore.

For backup and restore of Software versions lower than 2.5, please refer to the QIAcuity Software Suite Backup and Restore Scripts for version 2.0, 2.1.7, 2.1.8 and 2.2. 

Note: Windows Admin permission is needed to setup and perform an automated backup and for manual backup and restore.

Please read the QIAcuity Software Suite Backup and Restore Scripts document for more information and instructions how to use the scripts.

 

QIAcuity Control Software
SOFTWARE (322MB)

Version 2.5

 

The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis.

The new version 2.5 of the QIAcuity CSW offers a configurable auto logoff times which enables users to turn off or define logoff times per instrument. In addition the new version supports assay development by providing an essential temperature gradient functionality.

Furthermore improvements were implemented, for example, for the accuracy of time estimation for various software steps.

Detailed information about the QIAcuity Control Software version 2.5 is available in the Release Note, which can also be downloaded under section Software Release Notes.

 

Note: The latest CSW version 2.5 is only compatible with the Software Suite version 2.5. If only one software component is updated, no connection between the Software Suite and the CSW can be established.

Important: Please follow the instructions provided in the QIAcuity User Manual for software version 2.5 and in the Release Note.

It is strongly recommended to update the QIAcuity Software Suite first before proceeding with the QIAcuity Control Software!

Note: After clicking reboot during CSW upgrade or change of Suite connection, the login screen may appear for short period. Please ignore it and wait for the QIAcuity instrument to shut down and restart itself.

 

Please contact QIAGEN Technical Services if you are unsure and require technical support.


 
 

Application Notes (12)
Here, we present a highly efficient, high-throughput workflow that combines two technologies, cellenONE and QIAcuity Digital PCR, to accurately analyze miRNAs in well-defined individual cells and populations of cells.
This study tested a workflow for quantitation and qualification of AAV samples using a duplex assay on the QIAcuity dPCR instrument targeting both an insert (GFP) and the viral backbone (AAV2-ITR). With very low intra-assay and inter-assay CVs <6.5%, we demonstrate one of the main benefits of dPCR: reproducibility.
Here, we present a workflow that combines two technologies, cellenONE and QIAcuity Digital PCR, which accelerate and streamline high-throughput analyses of target copy numbers in cultured cells. The workflow starts with detecting and sorting defined populations of cells as well as individual cells using cellenONE, followed by multiplexing dPCR on the QIAcuity platform. Copy number variations of target regions are then analyzed using the QIAcuity Software Suite, providing an intuitive and fast interpretation of results.
With the cellenONE-QIAcuity workflow, we achieved high-throughput absolute quantification of low-abundance targets at the single-cell level. cellenONE’s single-cell isolation platform enabled real-time and 100% accurate single-cell isolation without compromising viability and transcript expression. This allowed us to use the exact number of intact cells for RT-dPCR reactions. Moreover, the elimination of the RNA purification step significantly reduced hands-on time, and QIAcuity’s probe-based chemistry allowed the multiplexing of up to five targets in a one-step RT-dPCR format with no or minimal optimization.
Here we report the use of saliva samples in combination with dPCR as a suitable alternative to screen for individuals infected with SARS-CoV-2.
The QIAgility instrument is a liquid handler designed for automating PCR setup. For compatibility with the QIAcuity, we developed an adapter to secure up to two nanoplates onto the deck of the QIAgility. Using the QIAgility software, we have optimized a protocol that works for all nanoplate types and QIAcuity applications. Here we report the performance of a front end automated QIAgility dPCR nanoplate setup procedure for use with the QIAcuity dPCR system.
The QIAcuity digital PCR system combined with the QIAcuity One-Step Viral RT-PCR Kit enables precise detection and quantitation of vector-borne viruses in mosquitoes. The results presented in this comparison study showed that digital PCR is a powerful tool for absolute quantitation of low abundant targets and is a more reliable detection method than qPCR. Multiplexing allows detection and quantitation of multiple targets in a single reaction more efficiently by increasing sample throughput at a reduced cost per target.
The goal of this work was to compare performance of quantitative PCR (qPCR) and digital PCR (dPCR) in the quantification of gene expression and Wolbachia abundances in Nasonia parasitoid wasps.
Here we provide an integrated rAAV genome titer method using the QIAcuity Digital PCR (dPCR) System with detailed parameters for high assay performance. Using this optimized method for pre-PCR handling of in-process rAAV samples, the results demonstrated that QIAcuity dPCR system generates the same level of accuracy and precision as the current gold standard ddPCR system but with much faster sample-to-result times (2 hours vs 7 hours) and higher overall throughput and scalability.
Here we compared the performance of qPCR and the nanoplate dPCR techniques. The digital PCR method on the QIAcuity significantly improved precision when measuring copy number states and sensitivity of mutation detection through absolute quantification and reduced standard error. This is advantageous in various applications, including copy number variation analysis, small fold-change and rare mutation detection.
Here we demonstrate how to optimize your assays on a microfluidic nanoplate-based digital PCR system, the QIAcuity, and provide recommendations for a seamless transfer. Moreover, the QIAcuity dPCR workflow is very similar to qPCR.
Q-Solution is an innovative and versatile PCR additive that works in part by lowering the melting temperature of DNA. Adjusting template melting properties can be useful when interrogating DNA with high-GC content or with complex secondary structures, such as adeno-associated virus (AAV) genomes. Q-Solution has additional properties that can be used to mitigate the carryover of various PCR inhibitors. This application note will highlight a few examples of how the Q-Solution Kit can be used in QIAcuity workflows.
Brochures & Guides (4)
A versatile workflow for the detection of low-abundance microbes
Fast. Scalable. Reliable.
Previous Software Versions (5)

Version 2.1.8

 

The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. This software is also used for the configuration of the system and provides access to the QIAcuity user management. The QIAcuity Software Suite is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments.  For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).

The new version 2.1.8 of the QIAcuity software (QIAcuity Software Suite version 2.1.8 and QIAcuity CSW version 2.1.8.) offers bug fixes for repeated network connection issues and improved error handling. In addition, it fixes issues seen with latest versions of Microsoft Edge and Google Chrome browsers as well as issues seen with read-only plates after a software update.

Detailed information about the QIAcuity Software Suite version 2.1.8 is available in the Release Note, which can also be downloaded under section “Software Release Notes”.

Important: Please ensure that updating to QIAcuity Software Suite version 2.1.8 is performed by the same Windows admin user using the same Windows login name that installed the previous QIAcuity Software Suite version. In case you cannot use the same Windows login name or the software update resulted in the browser error message "Can't reach this page" please contact our Technical Services for additional instructions.

Note: The latest Software Suite version 2.1.8 is only compatible with the latest Control Software (CSW) version 2.1.8. If only one software component is updated, no connection between the Software Suite and the CSW can be established.

Important: Please follow the instructions provided in the QIAcuity User Manual for software version 2.1 and in the Release Note. It is strongly recommended to update the QIAcuity Software Suite first before proceeding with the QIAcuity Control Software!

Please contact QIAGEN Technical Services if you are unsure and require technical support.

 

 SHA1 checksum: DE2416D926BB98D82A332E7B25EF66728F120DDA

 

 

Version 2.2

 

The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. This software is also used for the configuration of the system and provides access to the QIAcuity user management. The QIAcuity Software Suite is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).


The new version 2.2 of the QIAcuity software (QIAcuity Software Suite v 2.2 and QIAcuity CSW v 2.2.) offers improvements for users working under GMP by adding a user ID validation during the report signing and the addition of timezone offset stamp for audit trail entries and for result report data. Furthermore, the addition of a standard deviation and coefficient of variance calculation in percentage of mean concentration calculation for replicates where implemented. In addition, the instrument camera stability was improved and an internal validation method was implemented.

 

Detailed information about the QIAcuity Software Suite version 2.2 is available in the Release Note, which can also be downloaded under section “Software Release Notes”.

Note: The latest Software Suite version 2.2 is only compatible with the latest Control Software (CSW) version 2.2. If only one software component is updated, no connection between the Software Suite and the CSW can be established.

 

Important: Please follow the instructions provided in the QIAcuity User Manual for software version 2.2 and in the Release Note.

 

It is strongly recommended to update the QIAcuity Software Suite first before proceeding with the QIAcuity Control Software. 

 

Please contact QIAGEN Technical Services if you are unsure and require technical support.

 

SHA1 checksum: 63A1689F2EB557809F418D159DA438FDC8B327A3

 

 

Version 2.1.8

 

The QIAcuity Control Software (CSW) is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis.

The new version 2.1.8 of the QIAcuity software (QIAcuity Software Suite version 2.1.8 and QIAcuity CSW version 2.1.8) offers bug fixes for repeated network connection issues and improved error handling. In addition, it fixes issues seen with latest versions of Microsoft Edge and Google Chrome browsers as well as issues seen with read-only plates after a software update.

Detailed information about the QIAcuity Control Software version 2.1.8 is available in the Release Note, which can also be downloaded under section “Software Release Notes”.

 

Note: The latest CSW version 2.1.8 is only compatible with the latest Software Suite version 2.1.8. If only one software component is updated, no connection between the Software Suite and the CSW can be established.

Important: Please follow the instructions provided in the QIAcuity User Manual for software version 2.1 and in the Release Note. It is strongly recommended to update the QIAcuity Software Suite first before proceeding with the QIAcuity Control Software!

 

Please contact QIAGEN Technical Services if you are unsure and require technical support.

 

 

SHA1 checksum: D3982B419D7C4CF39FBDDDAA4C0D351B39398278

QIAcuity Software Suite
SOFTWARE (389MB)

Version 1.2

A newer version of the Software Suite is available. Please use this version for the update of older plates if required.



The QIAcuity Software Suite 1.2 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).

The following browsers are supported in the QIAcuity Software Suite:

-Mozilla Firefox (version 64.0.2 or higher)
-Microsoft Edge (version 44.17763.1.0 or higher)
-Google Chrome (version 71.0.3578.98 or higher)

The new QIAcuity Software Suite 1.2 offers a functionality that enables users of the QIAcuity Software 1.1.3 to upgrade to the new version while keeping the library of previously stored plate runs.

Note: If you have exported plates from QIAcuity Software Suite 1.1.3 that you would like to import and use in QIAcuity Software Suite 1.2, you will need to import the plates before upgrading from version 1.1.3 to version 1.2. You may then export the plates again. Future software version starting from QIAcuity Software Suite 2.0 will facilitate import of plates from previous QIAcuity Software Suite versions.

The new improvements are as follows:

-Support for the Nanoplate 8.5k 24-well
-Hyperwell functionality to combine several wells to one combined well for analysis
-Automated plate archiving functionality
-Functionality to show the number of single/double positives in 2D scatterplots
-VPF (Volume Precision Factor) to further improve concentration calculation (see related resources)
-Additional improvements for stabilization and troubleshooting

Version 2.2

 

The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis.

 

The new Version 2.2 of the QIAcuity software (QIAcuity Software Suite v 2.2 and QIAcuity CSW v 2.2.) offers improvements for users working under GMP by adding a user ID validation during the report signing and the addition of timezone offset stamp for audit trail entries and for result report data. Furthermore, the addition of a standard deviation and coefficient of variance calculation in percentage of mean concentration calculation for replicates where implemented. In addition, the instrument camera stability was improved and an internal validation method was implemented.

 

Detailed information about the QIAcuity Control Software version 2.2 is available in the Release Note, which can also be downloaded under section “Software Release Notes”.

 

Note: The latest CSW version 2.2 is only compatible with the Software Suite version 2.2. If only one software component is updated, no connection between the Software Suite and the CSW can be established.

 

Important: Please follow the instructions provided in the QIAcuity User Manual for software version 2.2 and in the Release Note.

 It is strongly recommended to update the QIAcuity Software Suite first before proceeding with the QIAcuity Control Software.
 

 

Note: After clicking reboot during CSW upgrade or change of Suite connection, login screen may appear for short period. Please ignore it and wait for the QIAcuity instrument to shut down and restart itself.


Please contact QIAGEN Technical Services if you are unsure and require technical support.

 

 

SHA1 checksum: 3892D7A434A8F072A15008C76EB088BB78F1C255

Technical Information (2)

Compliance with 21 CFR Part 11 involves both technical (i.e., hardware and software) and procedural requirements. This document explains how the QIAcuity system contributes to fulfilling those technical requirements.

A new temperature gradient functionality to determine the optimal primer annealing temperature of a digital PCR assay


Safety Data Sheets (1)
Certificates of Analysis (1)
Technical Information and Important Notes (1)

FAQ

Can I use the QIAcuity Nanoplate in more than one runs?

The plate is designed for a single use run. For example, even if only 30 samples are loaded into the 96-well plate, a whole plate will be sealed by the roller. It can't be unsealed and used for another run. The QIAcuity Software won’t allow to set up a separate experiment for the same nanoplate to avoid that previously processed plates being not partitioned a second time.

3781
What are the storage conditions and expiry date of QIAcuity consumables?
The QIAcuity Probe PCR Kit should be stored immediately upon receipt at –30 to –15°C in a constant-temperature freezer and protected from light. The QIAcuity Probe PCR master mix can also be stored protected from light at 2–8°C. Components are stable for 12 months, unless otherwise indicated on the label. 
The QIAcuity EG PCR Kit should be stored immediately upon receipt at –30 to –15°C in a constant-temperature freezer and protected from light. The QIAcuity EG PCR master mix can also be stored protected from light at 2–8°C. Components are stable for 6 months, unless otherwise indicated on the label. 
The QIAcuity Nanoplates does not have expiry date and are stable for at least 1 year when stored at RT. 
FAQ ID - 9214
Where can I find assays specifically designed for QIAcuity?
QIAGEN dPCR assays (such as dPCR LNA Mutation Assays) can be found on https://geneglobe.qiagen.com/.
FAQ ID - 9207
Can I prepare a dPCR reaction directly in QIAcuity Nanoplate?

A standard PCR plate is required to set up dPCR reaction before transferring it to the nanoplate to ensure a proper mixing of the reaction mix before partitioning. 

3774
How can I get the latest VPF (Volume Precision Factor)?
The up-to-date VPF can be downloaded in the resource section of the QIAcuity product webpage. The VPF  is compatible with the QIAcuity Software Suite 1.2 and higher. The latest VPF file contains all factors for all existing nanoplate batches. If a nanoplate from a new nanoplate batch is run and the latest version of the VPF was not installed, the software will recognize this and will give a message to install the latest VPF file. Please note that Applying the VPF file cannot be reversed.
FAQ ID - 9206
What is the VPF (Volume Precision Factor)?
The VPF provides a set of well-specific and molding form-specific factors used to specify the exact reaction volume of a nanoplate, thus increasing the concentration calculation of each well. 
FAQ ID - 9218
Where can I find assays specifically designed for QIAcuity?

QIAGEN dPCR assays (such as dPCR LNA Mutation Assays) can be found on https://geneglobe.qiagen.com/.

3773
Is it possible to change voltage set-up from 110V to 230V on the QIAcuity instruments?

This is not needed. The QIAcuity is equipped with a flexible power supply technology and operates within a range of 100–240V AC, 50/60 Hz, 1500 VA (max). 

3761
What is the impact of not applying the latest VPF? Can I reanalyze previously obtained results after installing the latest VPF?

If you had run a nanoplate for which the installed VPF misses the specific factor, the software will notify you. If you then analyze without the specific VPF, the impact depends on the variation of the partition volume of the new Nanoplate batch compared to the latest. Typically this variation is ±6–7% (approx. 5% CV over the entire plate). The analysis may be repeated after updating the VPF file. After installing the latest VPF and re-analysis of the run, a copy of the plate is generated in the QIAcuity Software Suite including the new results. 

3769
Can I use a custom master mix instead of a QIAGEN master mix?

QIAGEN master mixes are optimized for nanoplate microfluidics and are recommended to be used with our dPCR system. They also include an optimized reference dye required for proper analysis.

3777
Does an incorrect handling of a plate might affect the results?
The QIAcuity reads emitted fluorescence from the bottom of the plate, which is covered with a foil. For best results, keep the foil clean and avoid damages such as scratches. Also, keep the barcode on the side of the plate clean and intact. Ensure that you wear gloves when working with a plate and do not apply force to it. For a safe handling of the plate, please  place the plate into a nanoplate tray.
FAQ ID - 9202
Can I use a custom master mix instead of a QIAGEN master mix?
QIAGEN master mixes are optimized for nanoplate microfluidics and are recommended to be used with our dPCR system. They also include an optimized reference dye required for proper analysis.
FAQ ID - 9211
Can I optimize a dPCR assay on the QIAcuity using gradient temperature?

An essential temperature gradient functionality was introduced with software version 2.5. When updating older software versions to 2.5, each QIAcuity instrument will offer the temperature gradient and may be used to find the best cycling temperature for new dPCR assays. When running a QIAcuity Four or a QIAcuity Eight, all plates may have their own temperature profile, including the option for a temperature gradient.

FAQ ID - 3783
Which sample preparation method is recommended for QIAcuity dPCR?
QIAGEN offers a complete range of nucleic acid purification systems. These include QIAprep kits for purification of plasmid DNA, QIAamp and DNeasy kits for purification of genomic DNA, RNeasy kits for purification of total RNA, and the PAXgene Blood RNA System for stabilization and purification of RNA from blood. Phenol and other contaminants can be efficiently removed from crude RNA preps using the RNeasy MinElute Cleanup Kit to clean up and concentrate RNA for sensitive assays. Details about QIAGEN kits for nucleic acid purification can be found at www.qiagen.com.
FAQ ID - 9213
Can I use an empty nanoplate without seal to perform a dry run?
The QIAcuity instrument software does not allow to read and process a plate without seal. If you would like to perform a dry run please use sealed plate and set up this plate in the QIAcuity Software Suite. 
FAQ ID - 9201
Can I use the dPCR master mix in a qPCR cycler for optimization purposes?
dPCR master mix can be used in qPCR to optimize sample concentration and/or primer/probe concentration prior to assay transfer from qPCR to dPCR. 
FAQ ID - 9209
Is it possible to change voltage set-up from 110V to 230V on the QIAcuity instruments?
This is not needed. The QIAcuity is equipped with a flexible power supply technology and operates within a range of 100–240V AC, 50/60 Hz, 1500 VA (max). 
FAQ ID - 9195
How are results stored within the QIAcuity Software Suite?
Results are stored as part of the plates within the QIAcuity Software Suite. In version 1.1.2, plates can be exported to another file location, for example, an external HDD, and imported again if needed. From version 1.2 onwards, plates can also be archived automatically. To do so, an archive destination has to be defined. Additional information can be found in the user manual. 
FAQ ID - 9198
Which sample preparation method is recommended for QIAcuity dPCR?

QIAGEN offers a complete range of nucleic acid purification systems. These include QIAprep kits for purification of plasmid DNA, QIAamp, and DNeasy kits for purification of genomic DNA, RNeasy kits for purification of total RNA, and the PAXgene Blood RNA System for stabilization and purification of RNA from blood. Phenol and other contaminants can be efficiently removed from crude RNA preps using the RNeasy MinElute Cleanup Kit to clean up and concentrate RNA for sensitive assays. Details about QIAGEN kits for nucleic acid purification can be found at www.qiagen.com.

3779
Is it necessary to reanalyze a plate with VPF (Volume Precision Factor) that was already processed using a QIAcuity instrument that was purchased in 2020?

If you had analyzed your nanoplates without VFP, the impact depends on the variation of the partition volume of the new nanoplate batch compared to the latest. The VPF reduces the CV from approximately 5% to 2%. We recommend to reanalyze results in case the data originated from different wells (e.g., copy number variations or gene expression data sets for which the reference sample was measured in a different well). Results obtained across different plates should also be r-analyzed. A reanalysis is not required for assay data that were analyzed within the same well (e.g., mutation rate determination using two channels within the same well).

3771
Is a standard curve needed in dPCR?

In dPCR we measure the absolute concentration of targets at endpoint reaction. Concentrations of unknowns can be determined based on dPCR results observed (number of negatives, number of positives, and partition volume analyzed).

3786
How can I upgrade the software on the instrument (CSW) and the QIAcuity Software Suite?
Both software are designed to be upgraded by users. The user manual includes instructions on how to perform the upgrades; instructions for the QIAcuity Suite Software upgrade can be found at page 38 and instructions for the CSW upgrade on page 67 (QIAcuity User Manual, 03/2021).  
FAQ ID - 9196
Is it necessary to reanalyze a plate with VPF (Volume Precision Factor) that was already processed using a QIAcuity instrument that was purchased in 2020?
If you had analyzed your nanoplates without VFP, the impact depends on the variation of the partition volume of the new nanoplate batch compared to the latest. The VPF reduces the CV from approximately 5% to 2%. We recommend to re-analyze results in case the data originated from different wells (e.g., copy number variations or gene expression data sets for which the reference sample was measured in a different well). Results obtained across different plates should also be re-analyzed. A re-analysis is not required for assay data that were analyzed within the same well (e.g., mutation rate determination using two channels within the same well).
FAQ ID - 9205
When and how often do I need a new VPF (Volume Precision Factor)?

 In general, nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. If a new molding form is used for nanoplate manufacturing, potential variation of partition sizes can be addressed by applying the molding form-specific VPF. Thus, each time a new molding form is used, a new VPF is created and made available. Currently, the VPF is updated once every 3–6 months.

3785
When and how often do I need a new VPF (Volume Precision Factor)?
 In general, nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. If a new molding form is used for nanoplate manufacturing, potential variation of partition sizes can be addressed by applying the molding form-specific VPF. Thus, each time a new molding form is used, a new VPF is created and made available. Currently, the VPF is updated once every 3–6 months.
FAQ ID - 9219
What is the input volume for QIAcuity Nanoplate well?
Nanoplate 26K 24-well: 40 μl 
Nanoplate 8.5K 24-well:12 μl 
Nanoplate 8.5K 96-well: 12 μl 
FAQ ID - 9216
What is the purpose of the reference channel in QIAcuity?
The fluorescent signal in the reference channel is measured to determine the number of valid partitions in a well. In addition, differences in the signal intensities between partitions are normalized and the fluorescent signals in the target channels are corrected accordingly.
FAQ ID - 9210
Is a standard curve needed in dPCR?
In dPCR, we measure the absolute concentration of targets at endpoint reaction. Concentrations of unknowns can be determined based on dPCR results observed (number of negatives, number of positives, and partition volume analyzed).
FAQ ID - 9220
What is the purpose of the reference channel in QIAcuity?

The fluorescent signal in the reference channel is measured to determine the number of valid partitions in a well. In addition, differences in the signal intensities between partitions are normalized and the fluorescent signals in the target channels are corrected accordingly.

3776
What is the VPF (Volume Precision Factor)?

The VPF provides a set of well-specific and molding form-specific factors used to specify the exact reaction volume of a nanoplate, thus increasing the concentration calculation of each well. 

3784
Can I see on the QIAcuity Software Suite report file if the VPF (Volume Precision Factor) has been used or not?

Yes, the report includes a notification if the matching VPF was missing and, therefore, not applied to the analysis. If the matching VPF was applied there is no notification on the report. 

3770
What are the storage conditions and expiry date of QIAcuity consumables?

The QIAcuity Probe PCR Kit should be stored immediately upon receipt at –30 to –15°C in a constant-temperature freezer and protected from light. The QIAcuity Probe PCR master mix can also be stored protected from light at 2–8°C. Components are stable for 12 months, unless otherwise indicated on the label. 
The QIAcuity EG PCR Kit should be stored immediately upon receipt at –30 to –15°C in a constant-temperature freezer and protected from light. The QIAcuity EG PCR master mix can also be stored protected from light at 2–8°C. Components are stable for 6 months, unless otherwise indicated on the label. 
The QIAcuity Nanoplates does not have expiry date and are stable for at least 1 year when stored at RT. 

3780
What is the input volume for QIAcuity Nanoplate well?

Nanoplate 26K 24-well: 40 μl 
Nanoplate 8.5K 24-well:12 μl 
Nanoplate 8.5K 96-well: 12 μl 

3782
How to prepare DNA prior to dPCR?

All DNA samples used in reaction mixes should show similar quality and quantity, which can easily be assessed using UV spectrophotometry. DNA samples with an average length of ≥20 kb (e.g., genomic DNA purified via spin column with silica membrane) should be fragmented by restriction digestion before partitioning. Enzymatic fragmentation of larger DNA ensures an even distribution of template throughout the QIAcuity Nanoplate, which in turn leads to an accurate and precise quantification.

3778
Can I see error codes on the instrument touchscreen?

The instrument software GUI shows error codes including a description and information how to resolve the error. The instrument touchscreen shows an alarm icon in the upper right corner that turns red in case of an instrument failure. Accessing the System Status in the Tool tab allows users to clear errors. Rebooting of the instrument is required to complete the removal of the error.  Please do not skip this step. You may always contact QIAGEN Technical Services in case of any question. 

3763
Can I use the QIAcuity Nanoplate in more than one runs?
The plate is designed for a single use run. For example, even if only 30 samples are loaded into the 96-well plate, a whole plate will be sealed by the roller. It can't be unsealed and used for another run. The QIAcuity Software won’t allow to set up a separate experiment for the same nanoplate to avoid that previously processed plates being not partitioned a second time.
FAQ ID - 9215
What is the impact of not applying the latest VPF? Can I reanalyze previously obtained results after installing the latest VPF?
If you had run a nanoplate for which the installed VPF misses the specific factor, the software will notify you. If you then analyze without the specific VPF, the impact depends on the variation of the partition volume of the new Nanoplate batch compared to the latest. Typically this variation is ±6–7% (approx. 5% CV over the entire plate). The analysis may be repeated after updating the VPF file. After installing the latest VPF and re-analysis of the run, a copy of the plate is generated in the QIAcuity Software Suite including the new results. 
FAQ ID - 9203
Can I see on the QIAcuity Software Suite report file if the VPF (Volume Precision Factor) has been used or not?
Yes, the report includes a notification if the matching VPF was missing and, therefore, not applied to the analysis. If the matching VPF was applied there is no notification on the report. 
FAQ ID - 9204
How to prepare DNA prior to dPCR?
All DNA samples used in reaction mixes should show similar quality and quantity, which can easily be assessed using UV spectrophotometry. DNA samples with an average length of ≥20 kb (e.g., genomic DNA purified via spin column with silica membrane) should be fragmented by restriction digestion before partitioning. Enzymatic fragmentation of larger DNA ensures an even distribution of template throughout the QIAcuity nanoplate, which in turn leads to an accurate and precise quantification.
FAQ ID - 9212
Can I use an empty nanoplate without seal to perform a dry run?

The QIAcuity instrument software does not allow to read and process a plate without seal. If you would like to perform a dry run please use sealed plate and set up this plate in the QIAcuity Software Suite. 

3767
Can I use the dPCR master mix in a qPCR cycler for optimization purposes?

dPCR master mix can be used in qPCR to optimize sample concentration and/or primer/probe concentration prior to assay transfer from qPCR to dPCR. 

3775
What is the scope of a regular maintenance of the QIAcuity?

The user manual contains instructions on how to perform a regular cleaning and decontamination, and how to replace air filters on the QIAcuity instruments. A regular maintenance reduces the dust in the instrument and therefore minimizes the presence of dust particles on the nanoplate, which might interfere with the plate analysis.

3765
How can I get the latest VPF (Volume Precision Factor)?

The up-to-date VPF can be downloaded in the resource section of the QIAcuity product webpage. The VPF  is compatible with the QIAcuity Software Suite 1.2 and higher. The latest VPF file contains all factors for all existing nanoplate batches. If a nanoplate from a new nanoplate batch is run and the latest version of the VPF was not installed, the software will recognize this and will give a message to install the latest VPF file. Please note that Applying the VPF file cannot be reversed.

3772
Does an incorrect handling of a plate might affect the results?

The QIAcuity reads emitted fluorescence from the bottom of the plate, which is covered with a foil. For best results, keep the foil clean and avoid damages such as scratches. Also, keep the barcode on the side of the plate clean and intact. Ensure that you wear gloves when working with a plate and do not apply force to it. For a safe handling of the plate, please  place the plate into a nanoplate tray.

3768
Can I see error codes on the instrument touchscreen?
The instrument software GUI shows error codes including a description and information how to resolve the error. The instrument touchscreen shows an alarm icon in the upper right corner that turns red in case of an instrument failure. Accessing the System Status in the Tool tab allows users to clear errors. Rebooting of the instrument is required to complete the removal of the error.  Please do not skip this step. You may always contact QIAGEN Technical Service in case of any question. 
FAQ ID - 9197
Can the QIAcuity One 2plex be upgrade to the QIAcuity One 5plex?
No. The QIAcuity platform introduces four variations: QIAcuity One 2plex, QIAcuity One 5plex, QIAcuity Four (5plex), and QIAcuity Eight (5plex). All of them have fix channel combination. 
FAQ ID - 9200
Can the QIAcuity One 2plex be upgrade to the QIAcuity One 5plex?

No. The QIAcuity platform introduces four variations: QIAcuity One 2plex, QIAcuity One 5plex, QIAcuity Four (5plex), and QIAcuity Eight (5plex). All of them have fix channel combination. 

3766
Can I prepare a dPCR reaction directly in QIAcuity Nanoplate?
A standard PCR plate is required to set up dPCR reaction before transferring it to the nanoplate to ensure a proper mixing of the reaction mix before partitioning. 
FAQ ID - 9208
Can I optimize a dPCR assay on the QIAcuity using gradient temperature?
QIAcuity does not support temperature gradient in PCR cycling profile. However, optimization of a dPCR assay can be done by qPCR on a gradient cycler using the dPCR master mix and then transferred from qPCR to dPCR. 
FAQ ID - 9217
How are results stored within the QIAcuity Software Suite?

Results are stored as part of the plates within the QIAcuity Software Suite. In version 1.1.2, plates can be exported to another file location, for example, an external HDD, and imported again if needed. From version 1.2 onwards, plates can also be archived automatically. To do so, an archive destination has to be defined. Additional information can be found in the user manual. 

3764
How can I upgrade the software on the instrument (CSW) and the QIAcuity Software Suite?

Both software are designed to be upgraded by users. The user manual includes instructions on how to perform the upgrades; instructions for the QIAcuity Suite Software upgrade can be found at page 38 and instructions for the CSW upgrade on page 67 (QIAcuity User Manual, 03/2021).  

3762
What is the scope of a regular maintenance of the QIAcuity?
The user manual contains instructions on how to perform a regular cleaning, decontamination, and how to replace air filters on the QIAcuity instruments. A regular maintenance reduces the dust in the instrument and therefore minimizes the presence of dust particles on the nanoplate, which might interfere with the plate analysis.
FAQ ID - 9199