TopTaq DNA Polymerase

Read phase-out notice below
For highly reliable end-point PCR applications with unrivaled ease of use

Storage at 4°C allows immediate reaction setup
Convenient room-temperature handling
A single, preoptimized protocol suitable for all PCR assays
High yields of specifically amplified DNA

TopTaq DNA Polymerase combines a number of convenient and unique features to provide unrivaled ease of use and ensure high DNA yields in a wide range of PCR applications. The unique TopTaq Stabilizer included in the enzyme storage buffer allows storage of TopTaq DNA Polymerase at 4°C and reaction setup at room temperature without time-consuming thawing of reagents. CoralLoad Concentrate, which contains two gel-tracking dyes for easier pipetting visualization and immediate gel-loading of PCR products, is also provided. High yields of PCR products are achieved, even when amplifying a range of different sized products using the same Mg²⁺ concentrations and annealing temperatures.

IMPORTANT NOTE: This product is phasing-out until October 15, 2020, and afterward, available only until stocks last. Visit the product page of the successor kit to view improved features or to request a trial kit.

For more information and FAQs on this transition, visit: www.qiagen.com/PCRresource.

Specifications
Product Resources

Specifications

Features	Specifications
Applications	PCR, RT-PCR, DNA fingerprinting
dNTP's included	No
Enzyme activity	5' -> 3' exonuclease activity
Mastermix	No
Reaction type	PCR amplification
Real-time or endpoint	Endpoint
Sample/target type	Genomic DNA and cDNA
Single or multiplex	Single
Storage conditions	Fridge storage and room temperature setup
With/without hotstart	Without Hotstart

Product Resources

Brochures & Guides (3)

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	Analyzing Genetic Differences - (EN) Second edition — innovative tools	Show details
	(EN) - Maximizing PCR and RT-PCR success — Third Edition Addressing critical factors and new solutions	Show details
	Product Profile - TopTaq DNA Polymerase and TopTaq Master Mix	Show details

FAQs (9)

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	How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red? FAQ ID -1644	View
	What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits? FAQ ID -1738	View
	What is the fidelity of TopTaq DNA Polymerase? FAQ ID -1739	View
	Can TA/UA cloning be done with PCR products amplified with TopTaq DNA Polmyerase and TopTaq Master Mix Kits? FAQ ID -1740	View
	Which nucleotide analogs can be used with TopTaq DNA Polymerase? FAQ ID -1741	View
	What is the shelf-life of TopTaq DNA Polymerase and Master Mix? FAQ ID -1743	View
	Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications? FAQ ID -1744	View
	Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications? FAQ ID -1745	View
	Is TopTaq DNA Polymerase a hot-start polymerase? FAQ ID -1748	View

Kit Handbooks (1)

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	TopTaq PCR Handbook - (EN) For standard and specialized end-point PCR applications without the need for optimization	Show details

Technical Information (1)

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	(EN) - Maximizing end-point PCR success with QIAGEN's automatable PCR solutions	Show details

Safety Data Sheets (3)

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	MSDS TopTaq DNA Polymerase (250)
	MSDS TopTaq DNA Polymerase (1000)
	MSDS TopTaq DNA Polymerase (5000)