text.skipToContent text.skipToNavigation

TopTaq DNA Polymerase

Read phase-out notice below
For highly reliable end-point PCR applications with unrivaled ease of use

  • Storage at 4°C allows immediate reaction setup
  • Convenient room-temperature handling
  • A single, preoptimized protocol suitable for all PCR assays
  • High yields of specifically amplified DNA

TopTaq DNA Polymerase combines a number of convenient and unique features to provide unrivaled ease of use and ensure high DNA yields in a wide range of PCR applications. The unique TopTaq Stabilizer included in the enzyme storage buffer allows storage of TopTaq DNA Polymerase at 4°C and reaction setup at room temperature without time-consuming thawing of reagents. CoralLoad Concentrate, which contains two gel-tracking dyes for easier pipetting visualization and immediate gel-loading of PCR products, is also provided. High yields of PCR products are achieved, even when amplifying a range of different sized products using the same Mg2+ concentrations and annealing temperatures.


IMPORTANT NOTE: This product is phasing-out until October 15, 2020, and afterward, available only until stocks last. Visit the product page of the successor kit to view improved features or to request a trial kit.


For more information and FAQs on this transition, visit: www.qiagen.com/PCRresource.


Applications PCR, RT-PCR, DNA fingerprinting
dNTP's included No
Enzyme activity 5' -> 3' exonuclease activity
Mastermix No
Reaction type PCR amplification
Real-time or endpoint Endpoint
Sample/target type Genomic DNA and cDNA
Single or multiplex Single
Storage conditions Fridge storage and room temperature setup
With/without hotstart Without Hotstart

Product Resources

You are not authorized to download the resource

Kit Handbooks (1)
For standard and specialized end-point PCR applications without the need for optimization
Show details
fragment fix placeholder
Server Error
    Server currently not available

    An internal server error has occurred.
    We apologize for any inconvenience the error might have on your browsing experience on our web site.

    Use the links below to find the information you need.