TopTaq Master Mix Kit

Read phase-out notice below
For highly reliable end-point PCR with unrivaled ease of use

Ready-to-use master mix with minimal pipetting steps
Fridge storage eliminates thawing time
Reliable high-yield PCR performance
Convenient room-temperature handling
Optimized protocol suitable for all PCR assays

The TopTaq Master Mix Kit offers maximum convenience by combining all the benefits of specific TopTaq DNA Polymerase with the advantage of a ready-to-use master mix. It contains TopTaq DNA Polymerase, the unique TopTaq PCR Buffer that minimizes the requirement for optimization, and dNTPs. The unique TopTaq Stabilizer included in the enzyme storage buffer allows storage of TopTaq Master Mix at 4°C and reaction setup at room temperature, without time-consuming thawing of reagents. Providing all components in a ready-to-use master mix reduces pipetting steps, which lowers the risk of contamination. High yields of PCR product are achieved, even after storing the TopTaq Master Mix for extended periods of time at 25°C or 4°C.

IMPORTANT NOTE: This product is phasing-out until October 15, 2020, and afterward, available only until stocks last. Visit the product page of the successor kit to view improved features or to request a trial kit.

For more information and FAQs on this transition, visit: www.qiagen.com/PCRresource.

Specifications
Product Resources

Specifications

Features	Specifications
Applications	PCR, RT-PCR, DNA Fingerprinting
dNTP's included	Yes (in Master Mix)
Enzyme activity	5' -> 3' exonuclease activity
Mastermix	Yes
Reaction type	PCR amplification
Real-time or endpoint	Endpoint
Sample/target type	Genomic DNA and cDNA
Single or multiplex	Single
Storage conditions	Fridge storage and room temperature setup
With/without hotstart	Without Hotstart

Product Resources

Brochures & Guides (3)

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	Analyzing Genetic Differences - (EN) Second edition — innovative tools	Show details
	(EN) - Maximizing PCR and RT-PCR success — Third Edition Addressing critical factors and new solutions	Show details
	Product Profile - TopTaq DNA Polymerase and TopTaq Master Mix	Show details

FAQs (10)

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	How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red? FAQ ID -1644	View
	What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits? FAQ ID -1738	View
	What is the fidelity of TopTaq DNA Polymerase? FAQ ID -1739	View
	Can TA/UA cloning be done with PCR products amplified with TopTaq DNA Polmyerase and TopTaq Master Mix Kits? FAQ ID -1740	View
	Which nucleotide analogs can be used with TopTaq DNA Polymerase? FAQ ID -1741	View
	What is the shelf-life of TopTaq DNA Polymerase and Master Mix? FAQ ID -1743	View
	Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications? FAQ ID -1744	View
	Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications? FAQ ID -1745	View
	Is TopTaq DNA Polymerase a hot-start polymerase? FAQ ID -1748	View
	Can TopTaq Master Mix simply be stored at room temperature on the lab bench for up to 4 months? FAQ ID -2052	View

Kit Handbooks (1)

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	TopTaq PCR Handbook - (EN) For standard and specialized end-point PCR applications without the need for optimization	Show details

Technical Information (1)

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	(EN) - Maximizing end-point PCR success with QIAGEN's automatable PCR solutions	Show details

Safety Data Sheets (1)

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	MSDS TopTaq Master Mix Kit (250)