How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System?
FAQ ID -1598

For detecting miRNA generated by the miScript System, 0.5 ng–1 ng cDNA per PCR is sufficient. Therefore cDNA made with 1 µg input RNA is usually adequate to perform miRNA profiling using all available miRNA miScript Primer Assays.

For mRNA detection, 5–10 ng cDNA is recommended.