What is the composition of Buffer ER? Is it available separately?

EndoFree Plasmid Kits provide anion-exchange-based endotoxin-free plasmid DNA purification. QIAfilter Cartridges enable fast lysate clearing by filtration. The purified DNA exceeds the purity obtained by 2 x CsCl gradient centrifugation and is suitable for advanced transfection-grade applications. The EndoFree Plasmid Buffer Set can be used for preparations of 10 mega or 5 giga transfection-grade plasmid or cosmid DNA preparations. The Buffer ER in EndoFree Plasmid Kits has been updated to exclude Triton™ X-114, ensuring REACH compliance and even lower endotoxin levels (<0.04 EU/μg) in line with FDA vaccine standards. The improved buffer reduces incubation time from 30 minutes on ice to just 5 minutes at room temperature.* *Please note: This update includes adjusted Buffer ER volumes and revised incubation times in the protocol.