DNeasy Blood & Tissue Kits

For spin-column or 96-well extraction of total DNA from animal blood and tissues and from cells, yeast, bacteria, or viruses

Standardized method for a variety of sample types
High yields even from specialized samples
High-quality DNA
Optimized protocols for a range of starting materials
Spin-column and 96-well high-throughput formats

DNeasy Blood & Tissue Kits provide fast and easy silica-based DNA extraction without phenol or chloroform in convenient spin-column and 96-well-plate formats. Most samples can be directly lysed with proteinase K, eliminating the need for mechanical disruption and reducing hands-on time. Optimized protocols for specific sample types provide reproducible extraction of high-quality DNA for life science, genotyping, and veterinary pathogen research applications. Extraction of DNA using the DNeasy Blood & Tissue Kit can be automated on the QIAcube Connect.

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Product Details
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Cat No./ID: 69504

DNeasy Blood & Tissue Kit (50)

$171.00

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50 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)

Cat No./ID: 69506

DNeasy Blood & Tissue Kit (250)

$736.00

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250 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)

Cat No./ID: 69581

DNeasy 96 Blood & Tissue Kit (4)

$1,350.00

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For 4 x 96 DNA minipreps: 4 DNeasy 96 Plates, Proteinase K, Buffers, S-Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Elution Microtubes RS, Caps, 96-Well Plate Registers

Cat No./ID: 69582

DNeasy 96 Blood & Tissue Kit (12)

$3,813.00

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For 12 x 96 DNA minipreps: 12 DNeasy 96 Plates, Proteinase K, Buffers, S-Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Elution Microtubes RS, Caps, 96-Well Plate Registers

The DNeasy Blood & Tissue Kits is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

DNeasy Mini and 96 procedures.

Real-time PCR.

Borrelia sp. is a pathogen carried by ticks that causes Lyme disease in humans and other mammals. DNA was purified from ticks using the DNeasy Blood & Tissue Kit. Borrelia DNA was detected in real-time PCR using the artus Borrelia LC PCR Kit on the LightCycler system. The lowest positive curve corresponds to 5 copies per PCR.

High-quality DNA.

DNA was purified from the indicated animal tissues using the DNeasy Blood & Tissue Kit and analyzed by agarose gel electrophoresis. For each sample, 0.5 µg DNA was analyzed undigested (left) or 1 µg DNA was digested with EcoRI (right). M: lambda-HindIII.

Genotyping of horses.

DNA was purified from hair of 3 brown horses and analyzed for coat-color genotypes using the MSH and ASIP PCR systems. [A] MSH PCR products were digested with TaqI. Horses 1 and 3 were identified as Ee heterozygotes, carrying the recessive e allele for red coat color. [B] Analysis of ASIP PCR products identify horses 1 and 2 as Aa heterozygotes, carrying the recessive a allele for black coat color. +: Positive Ee aa control DNA from a black horse. -: Negative PCR control. M: markers.

High-throughput purification.

DNA was purified from 24 mouse tails and from 24 rat tails using the DNeasy 96 Blood & Tissue Kit. DNA was analyzed on a 1% agarose gel. M: lambda-HindIII.

Genotyping of pigs.

DNA was purified from pig ear punches using the DNeasy Blood & Tissue Kit and amplified using the Animaltype Pig PCR Amplification Kit from Biotype AG and QIAGEN HotStarTaq DNA Polymerase. The electropherogram shows the genetic fingerprint for 12 polymorphic tetranucleotide microsatellites and one marker for gender determination (SBH23).

Efficient 16plex PCR.

Genomic DNA was purified from human K562 cells using the DNeasy Blood & Tissue Kit. Multiplex PCRs were performed in duplicate using the QIAGEN Multiplex PCR Kit with the indicated amount of DNA and 16 pairs of primers. M: 100 bp ladder.

DNA yields.

DNA was purified from 100 µl whole blood using the DNeasy Blood & Tissue Kit. Goat: DNA was purified from 50 µl goat whole blood. Using more than 50 µl goat blood gave no significant increase in DNA yield. Chicken: DNA was purified from 5 µl chicken whole blood. Bird blood contains nucleated erythrocytes, giving higher DNA yields than mammalian blood.

Performance

The efficient DNeasy Blood & Tissue procedure enables high yields of total DNA from animal blood and tissue samples (see table "Typical DNA yields from animal tissues using DNeasy Blood & Tissue Kits" and figure "DNA yields"). Optimized protocols ensure high yields from nonstandard samples, such as animal hair (see figure "Genotyping of horses"), as well as cultured cells, fixed tissues, or Gram-positive and -negative bacteria. Specialized online protocols are available for DNA purification from yeast, insects, hair, and other sample types.

Typical yields from animal tissues using DNeasy Blood & Tissue Kits
Source	Amount	DNA(µg)
Mammalian blood	100 µl	3–6
Bird blood	5 µl	9–40
HeLa cells	2 x 10⁶	15–25
Liver	25 mg	10–30
Brain	25 mg	15–30
Kidney	25 mg	15–30
Spleen	10 mg	5–30
Mouse tail	1.2 cm (tip)	10–25
Rat tail	0.6 cm (tip)	20–40
Pig ear	25 mg	10–30
Horse hair	10 hairs	2–4
Fish fin	20 mg	10–20
Fish spawn (mackerel)	10 mg	5–10

DNeasy Blood & Tissue Kits simplify purification of DNA from a wide range of sample types, including animal species commonly encountered in life science, veterinary, and genotyping applications (see figure "High-quality DNA"). Purified DNA is free from PCR inhibitors, enabling sensitive detection in standard, multiplex (see figure "Efficient 16plex PCR"), and real-time PCR (see figure "Real-time PCR"). DNeasy Blood & Tissue Kits provide reliable results — from laboratory analysis of transgenic mice (see figure "High-throughput purification") to livestock breeding programs (see figure "Genotyping of pigs") and pedigree genotyping. Routine testing applications can be easily scaled up using the DNeasy 96 Blood & Tissue Kit (see figure "High-throughput purification").

Principle

DNeasy Blood & Tissue Kits are designed for rapid purification of total DNA (e.g., genomic, mitochondrial, and pathogen) from a variety of sample sources including fresh or frozen animal tissues and cells, blood, or bacteria.

The DNeasy membrane combines the binding properties of a silica-based membrane with simple microspin technology or with the QIAGEN 96-Well-Plate Centrifugation System. DNA adsorbs to the DNeasy membrane in the presence of high concentrations of chaotropic salt, which remove water from hydrated molecules in solution. Buffer conditions in DNeasy Blood & Tissue procedures are designed to enable specific adsorption of DNA to the silica membrane and optimal removal of contaminants and enzyme inhibitors.

Purification requires no phenol or chloroform extraction or alcohol precipitation, and involves minimal handling. This makes DNeasy Blood & Tissue Kits highly suited for simultaneous processing of multiple samples. For higher-throughput applications, the DNeasy 96 Blood & Tissue Kit enables simultaneous processing of 96 or 192 samples.

Procedure

Reliable silica-membrane technology, in convenient spin-column or 96-well formats, ensures fast and reproducible DNA purification, eliminating the need for organic extraction and alcohol precipitation (see flowchart "DNeasy Mini and 96 procedures"). Samples are first lysed using proteinase K. Buffering conditions are adjusted to provide optimal DNA-binding conditions and the lysate is loaded onto the DNeasy Mini spin column or the DNeasy 96 plate. During centrifugation, DNA is selectively bound to the DNeasy membrane as contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in two efficient wash steps and DNA is then eluted in water or buffer, ready for use.

DNeasy Mini spin columns are prepackaged in collection tubes and individually sealed, providing convenience and safety. The purification procedure using DNeasy Mini spin columns can be automated on the QIAcube. The DNeasy 96 Blood & Tissue Kit provides high-throughput processing in a 96-well format using the QIAGEN 96-Well-Plate Centrifugation System.

Applications

DNeasy Blood & Tissue Kits provide high-quality DNA, ready to use in all downstream assays, including applications in:

Life science research

Livestock breeding

Pedigree genotyping

Veterinary pathogen research

Routine applied testing

Specifications

Features	Specifications
Applications	PCR, real-time PCR, genotyping
Elution volume	100–200 µl
Format	96-well plate, spin column
Main sample type	Blood, tissue
Processing	Manual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	DNA
Sample amount	100 µl/25 mg
Technology	Silica technology
Time per run or per prep	20 minutes – 1 hour
Yield	6 µg/30 µg

Product Resources

FAQs (25)

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	How do I safely inactivate biohazardous flow-through material? FAQ ID -12	View
	When should carrier be used with the QIAamp DNA Mini or the DNeasy Blood & Tissue Kit? FAQ ID -100	View
	Do you have data showing effects of sample size on DNA yield and purity using the DNeasy 96 Blood & Tissue kit? FAQ ID -129	View
	Do you have a protocol for purification of total DNA from yeast? FAQ ID -1253	View
	Do you have a protocol for purification of total DNA from insects? FAQ ID -1254	View
	Do you have a protocol for purification of total DNA from crude lysates? FAQ ID -1255	View
	How can I precipitate genomic DNA using isopropanol? FAQ ID -2953	View
	303 - Can I use my own lysis buffer with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?	View
	How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit? FAQ ID -374	View
	Is the quality and size of DNA extracted with the DNeasy Blood & Tissue kit good enough to generate DNA-libraries for next generation sequencing? FAQ ID - 3517	View
	What is the expected yield of genomic DNA isolated from bacteria with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit? FAQ ID -632	View
	What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA? FAQ ID -728	View
	What is the composition of buffer AE? FAQ ID -730	View
	Do you have a protocol for isolation of genomic DNA from insects? FAQ ID -904	View
	Do you have a protocol for the isolation of genomic DNA from bone? FAQ ID -908	View
	Do you have a protocol for the isolation of genomic DNA from sperm? FAQ ID -909	View
	Do you have a protocol for Acetyl Cysteine (NALC) treatment of viscous samples? FAQ ID -913	View
	Do you have a protocol for isolation of genomic DNA from saliva and mouthwash? FAQ ID -917	View
	Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser? FAQ ID -924	View
	Do you have a protocol for the isolation of viral DNA from stool? FAQ ID -929	View
	Do you have a protocol for isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit? FAQ ID -931	View
	Do you have a protocol for purification of DNA from cultured animal cells using the DNeasy 96 Blood & Tissue Kit? FAQ ID -934	View
	3192 - Is QIAGEN Protease compatible with Buffer ATL?	View
	Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K? FAQ ID - 3362	View
	What is the shelf-life for QIAGEN Proteinase K (cat. no. 19131, 19133)? FAQ ID - 3447	View

Scientific Posters (1)

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	(EN) - Workflow for DNA purification from tough specimens	Show details

Supplementary Protocols (3)

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	Purification of total DNA from yeast using the DNeasy® Blood & Tissue Kit - (EN) This protocol is designed for purification of DNA from up to 5 x 10⁷yeast cells.	Show details
	Purification of total DNA from insects using the DNeasy® Blood & Tissue Kit - (EN) This protocol is designed for purification of DNA from up to 50 mg of insects, such as drosophila.	Show details
	Purification of total DNA from crude lysates using the DNeasy® Blood & Tissue Kit - (EN) This protocol is designed for purification of DNA from a 200 μl crude lysate.	Show details

Quick-Start Protocols (2)

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	DNeasy 96 Blood & Tissue Quick-start Protocol (EN)	Show details
	Quick-Start Protocol DNeasy Blood & Tissue Kit	Show details

User-Developed Protocols (8)

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	Purification of total DNA from compact animal bone using the DNeasy® Blood & Tissue Kit - (EN)	Show details
	Purification of total DNA from animal sperm using the DNeasy® Blood & Tissue Kit; protocol 1 - (EN)	Show details
	Purification of total DNA from animal sperm using the DNeasy® Blood & Tissue Kit; protocol 2 - (EN)	Show details
	Purification of total DNA from nails, hair, or feathers using the DNeasy® Blood & Tissue Kit - (EN)	Show details
	Purification of viral DNA from animal stool using the DNeasy® Blood & Tissue Kit - (EN)	Show details
	Purification of total DNA from viscous samples using the DNeasy® Blood & Tissue Kit with acetyl cysteine (NALC) pretreatment - (EN)	Show details
	Purification of total DNA from animal saliva using the DNeasy® Blood & Tissue Kit - (EN)	Show details
	Purification of total DNA from soft tissues using the TissueLyser and the DNeasy® Blood & Tissue Kit - (EN)	Show details

Kit Handbooks (1)

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	DNeasy Blood & Tissue Handbook For purification of total DNA from animal blood, animal tissue, rodent tails, ear punches, cultured cells, fixed tissue, bacteria, insects	Show details

Brochures & Guides (1)

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	Allprotect® Reagent and AllPrep® DNA/RNA/Protein Kit	Show details

Safety Data Sheets (5)

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	MSDS DNeasy 96 Blood and Tissue Kit (4)
	MSDS DNeasy 96 Blood and Tissue Kit (12)
	MSDS DNeasy Blood and Tissue Kit (50)
	MSDS DNeasy Blood and Tissue Kit (250)
	CofA

References

Product citations in scientific articles

See more details on Bioz

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