Cat. No. / ID: AM01-020
qPCR SG and Probe Mixes are ready-to-use, 2x concentrated master mix of TaqNova HS polymerase, which is a mixture of recombinant Taq polymerase expressed in E. coli and a particular monoclonal anti-Taq antibody.
The TaqNovaHS polymerase enables easy set-up of a hot-start PCR reaction at room temperature. The antibody binds reversibly to the enzyme, inhibiting polymerase activity at ambient temperatures. This prevents the extension of non-specifically annealed primers and primer dimers formed at low temperatures during PCR set-up. The antibody is released from the polymerase during the initial DNA denaturation step, thus providing total DNA polymerase activity during standard cycling conditions.
|DNase contamination||None detected|
Precisely optimized buffer components, reliable DNA polymerase and the appropriate selection of PCR enhancers provide a high performance, sensitivity and specificity over a broad dynamic range and give consistent results across all commonly used real-time PCR platforms.
qPCR Mix is extensively tested for its performance in different Real-Time PCR assays. It is free of deoxyribonuclease contamination.
This is used for applications such as: