Highly specific and sensitive detection of His-tagged proteins
Flexible detection using any steptavidin or avidin conjugate
In detection procedures, the Penta·His Conjugate binds to a protein's His tag and the complex is localized using avidin derivatives, such as streptavidin, which can be conjugated to fluorescent dyes (e.g., streptavidin-R-PE) or to other proteins (e.g., HRP, AP) used in detection procedures.
The Penta·His Biotin Conjugate is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Performance of Penta·His Biotin Conjugate
Five consecutive histidine residues
Negligible with crude E. coli, yeast, mammalian, or insect cell lysates
Stabilized stock solution
Dependent on application
The Penta·His Biotin Conjugate can be used in conjunction with any streptavidin or avidin conjugate, delivering high flexibility in assay development.
The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.
Penta·His Biotin Conjugates allow colorimetric, chromogenic, chemiluminescent, or fluorescent detection of His-tagged proteins in western and dot blot, ELISA and other immunoassay procedures, and fluorescence-activated cell sorting (FACS).