QIAamp DNA Blood BioRobot 9604 Kit

For automated purification of genomic and mitochondrial DNA from whole blood using the BioRobot 9604 workstation

Features

  • Rapid isolation of high-quality, ready-to-use DNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Removal of contaminants and inhibitors

Useful Resources

QIAamp DNA Blood BioRobot 9604 Kit (12)

Cat. No. / ID: 965162

For 12 x 96 DNA preps: 12 QIAamp 96 Plates, Buffers, QIAGEN Protease, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Caps
The QIAamp DNA Blood BioRobot 9604 Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

The QIAamp DNA Blood BioRobot 9604 Kit provides automated DNA purification on the BioRobot 9604 workstation using proven QIAamp silica-membrane technology. The fully automated procedure on the BioRobot 9604 workstation requires less than 2.5 hours, including bar code reading and complete process documentation, with no hands-on time during the run.

Performance

QIAamp 96-well plates provide well-to-well uniformity in DNA recovery and purity (see figures " Reproducible DNA yield" and " Reproducible DNA purity"), and PCR experiments show no cross-contamination between samples in adjacent wells (see figure " Cross-contamination-free purification for reliable PCR"). As many as 96 samples of human whole blood or other body fluids can be processed yielding purified DNA in under 2 hours with minimal hands-on time. QIAamp sample preparation technology is fully licensed.

The QIAamp 96 DNA Blood procedure on the BioRobot 9604 increases productivity and reliability. DNA purified using the QIAamp 96 DNA Blood procedure on the BioRobot 9604 is free of contaminants and enzyme inhibitors, and is ready for use in amplification, Southern blotting, or other enzymatic assays for

  • Genetic disease testing
  • Identity testing
  • Cancer screening
  • See figures

    Principle

    No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in three efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp technology yields genomic and mitochondrial DNA from blood and other body fluids ready to use in PCR and blotting procedures.

    Fresh and frozen whole blood with common anticoagulants, such as citrate, EDTA, and heparin, may be processed with additional equipment and the use of a special protocol available from QIAGEN Technical Services or your local distributor.

    Procedure

    The QIAamp DNA Blood BioRobot 9604 Kit simplifies isolation of DNA from blood and other body fluids with a fast automated 96-well–plate procedure. Using the BioRobot 9604 Workstation, the QIAamp DNA Blood BioRobot 9604 Kit processes up to 200 µl sample size, with preparation time of 96 samples in less than 2 hours (see " Protocol"). The automated procedure, including bar code reading and process documentation, needs about 5 minutes hands-on time, with a turnaround time between consecutive runs of about 10 minutes.  The typical yield is 3–6 µg per 200 µl healthy whole blood, with an elution volume of 200 µl.
    See figures

    Applications

    The QIAamp DNA Blood BioRobot 9604 Kit provides automated high-throughput DNA purification on the BioRobot 9604 using proven QIAamp technology. The kit processes 96-well plates for up to 96 preps. Sample types include:

    • Fresh or frozen whole blood
    • Serum
    • Bone marrow
    • Body fluids
    • Cell suspensions

    Supporting data and figures

    Specifications

    FeaturesSpecifications
    applicationsPCR, blotting
    forautomatedprocessingBioRobot 9604
    sampleamount200 µl
    mainsampletypeWhole blood
    elutionvolume200 µl
    processingAutomated
    purificationoftotalrnamirnapolyamrnadnaorproteinGenomic DNA, mitochondrial DNA
    format96-well plate
    timeperrunorperprep<2 hours
    technologySilica technology
    yield3–6 µg

    Resources

    Kit Handbooks (1)
    For purification of genomic DNA from whole blood buffy coat bone marrow body fluids lymphocytes cultured cells using the BioRobot 9604 workstation

    Publications

    Functional polymorphisms of the human multidrug-resistance gene: multiple sequence variations and correlation of one allele with P-glycoprotein expression and activity in vivo.
    Hoffmeyer S; Burk O; von Richter O; Arnold HP; Brockmöller J; Johne A; Cascorbi I; Gerloff T; Roots I; Eichelbaum M; Brinkmann U;
    Proc Natl Acad Sci U S A; 2000; 97 (7):3473-8 2000 Mar 28 PMID:10716719

    FAQ

    Are there important considerations for plasma generation and urine handling?

    It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

    Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

    Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

    FAQ ID - 3699
    What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?

    QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

    QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

    For more information on activity of QIAGEN Protease and Proteinase K in various buffers please click here.

    FAQ ID -761
    What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

    Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

    Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

    FAQ ID -728
    How do I safely inactivate biohazardous flow-through material?

    Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
    Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

    FAQ ID -12