In this presentation, Dr. Ina Scheuerpflug will share her expertise on the critical steps and factors you need to know for efficient and precise gene expression studies using real-time PCR technology. Ina will conclude with a discussion of the NEW QIAGEN solutions for real-time PCR, the QIAquant Instruments.

In this webinar, Ina will cover:

  • Introduction to gene expression analysis;
  • Critical steps for real-time PCR analysis;
  • Improved methods for cDNA synthesis;
  • Real-time PCR essentials:
    • PCR kinetic and CT/Cq = threshold cycle
    • Absolute and relative quantification methods
    • Endogenous reference genes
    • Factors influencing PCR specificity
    • Different amplicon detection methods
    • Fluorescent dyes – the principle
  • NEW Real-Time PCR solutions from QIAGEN.
About the speaker
Ina Scheuerpflug Ph.D,
Ina Scheuerpflug, PhD is a Global MarketDirector in Discovery Sciences at QIAGEN, and has written on a number of scientific publications in the scientific area and has focused at QIAGEN on gene expression and gene regulation on a global level. She received her PhD at the Max-Plank-Institut for Biology in 1996, studying bacterial pilus adhesion and the interaction to the human host receptor/signaling pathway. Inahasa keen interest and solid knowledge in the emerging importance of gene expression studies.
Date of recording:Monday, March 8, 2021
Duration:60 minutes
Infectious Disease