Ligation Calculator

This tool determines the mass of insert needed for various inserts:vector molar ratios within the range suitable for typical ligation reactions.

icon_0330_cc_gen_calculator-sCalculate TargetsEnter the data of the targets you are interested in and receive sequencing calculations
Insights

 

Required insert DNA mass [g] = vector DNA mass [g] x {insert DNA length / vector DNA length} x desired insert to vector molar ratio

Notes: Desired ratio is one from set {1, 2, 3, 5 ,7}

Insert length should be smaller than vector length

Read more

First, the insert and vector DNA is digested using restriction endonucleases to create DNA fragments with sticky (or blunt) ends. Next, the desired DNA fragments are isolated and subsequently joined followed by treatment with DNA ligase. Finally, the resulting intact vector is transformed into competent E. coli cells, and transformants are identified through suitable genetic selection methods. 

 

Want to know more about the step-by-step process of DNA ligation and its typical reaction? Learn more.

Discover enzymes that you can use for ligating DNAs.

 

Looking for more enzymes?

Discover high-quality products designed for various molecular biology applications.

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