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The Stoffel DNA Polymerase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention, or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Product for commercial supply
Cat. No. / ID: Not Applicable
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Features
- Produces maximum performance with improved reaction buffer formulation
- No exonuclease activity
- High thermostability with a half-life of 20 minutes at 97.5°C
- Amplifies fragments of up to 5 kb and leaves ‘A’ overhangs
- Produces extreme yields
Product Details
Stoffel DNA Polymerase is a 62.7 kDa recombinant fragment of thermostable Taq DNA polymerase isolated from Thermus aquaticus. It is recommended for a wide range of applications that require DNA synthesis in extremely high temperatures.
The thermostability of Stoffel DNA Polymerase is about twice as high as the TaqNova DNA Polymerase. It requires a higher MgCl2 concentration level and lower ionic strength for optimum enzymatic activity. The vast magnesium optimum for Nova Stoffel DNA Polymerase reduces the need for magnesium optimization experiments. It increases the ease of multiplex PCR optimization which is the simultaneous amplification of multiple targets in the same reaction.
The increased thermal stability of the TaqNova Stoffel may lead to superior amplification of excessively GC-rich templates and templates with secondary structure by allowing denaturation temperatures as high as 98°C.
It is supplied with 20 mM Tris-HCl (pH 8.0 at 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol.
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 72°C in a 50 μL reaction.
Performance
| Assay | Specification |
| Protein Purity | ≥95% |
| DNase contamination | None detected |
Principle
Stoffel fragment is encoded by a modified form of the Thermus aquaticus DNA polymerase gene, which has been inserted into an Escherichia coli host. The modified gene encodes a 540 amino acid fragment lacking the N-terminal 292 amino acid portion of the full-length TaqNova DNA Polymerase.
Procedure
Quality Control
Protein purity is determined using Coomassie Blue detection assay by SDS-PAGE analysis resulting in ≥95% purity.
DNase contamination is evaluated through extensive PCR reactions.
Applications
This is used for applications such as:
- Amplification of short and medium-size sequences
- Diagnostic and multiplex PCR
- Genotyping – shows great performance in genetic mapping using primers with arbitrary sequences (RAPD)
- Allele-Specific Amplification PCR (ASA PCR) – amplification depends on 3’ terminal bases complementing the primer
Resources
Safety Data Sheets (1)