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QIAcuity Digital PCR System

For nanoplate-based digital PCR applications
  • Fully integrated system
  • Scalable format (1-, 4- and 8-plate instruments)
  • Advanced multiplexing capabilities (up to 5 plex)
  • Flexible sample throughput
  • Comprehensive results in under 2 hours

The QIAcuity Digital PCR System is designed to deliver precise and multiplexed quantification results for mutation detection, copy number variation (CNV), gene expression studies, gene-editing analysis, and many more. This nanoplate-based system seamlessly integrates a standard dPCR workflow of partitioning, thermocycling and imaging into a walk-away automated platform with minimal hands-on time.

 

The system is used in conjunction with the QIAcuity Nanoplates and Accessories.

 

Explore the virtual demo to learn more about QIAcuity.

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Cat No./ID: 911001
QIAcuity One, 2plex Device

One-plate digital PCR instrument for detecting up to 2 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts also included.

Cat No./ID: 911002
QIAcuity One, 2plex System

One-plate digital PCR instrument for detecting up to 2 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, and 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts also included.

Cat No./ID: 911006
QIAcuity One, 2plex System FUL-2

One-plate digital PCR instrument for detecting up to 2 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Full Agreement for 2 years with a 2 business day response time, and 2 preventive maintenance visits.

Cat No./ID: 911021
QIAcuity One, 5plex Device

One-plate digital PCR instrument for detecting up to 5 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts also included.

Cat No./ID: 911022
QIAcuity One, 5plex System

One-plate digital PCR instrument for detecting up to 5 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, and 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts also included.

Cat No./ID: 911026
QIAcuity One, 5plex System FUL-2

One-plate digital PCR instrument for detecting up to 5 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Full Agreement for 2 years with a 2 business day response time, and 2 preventive maintenance visits.

Cat No./ID: 911042
QIAcuity Four Platform System

Four-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, and 1 preventive maintenance visit.1 year warranty on labor, travel, and parts.

Cat No./ID: 911046
QIAcuity Four Platform System FUL-2

Four-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Full Agreement for 2 years with a 2 business day response time, and 2 preventive maintenance visits.

Cat No./ID: 911052
QIAcuity Eight Platform System

Eight-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, nanoplate roller, USB flash memory and QIAcuity Software Suite: includes installation, training, and 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts.

Cat No./ID: 911056
QIAcuity Eight Platform System FUL-2

Eight-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, nanoplate roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Full Agreement for 2 years with a 2 business day response time, and 2 preventive maintenance visits.

The QIAcuity is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease. Therefore, the performance characteristics of the product for clinical use (i.e., diagnostic, prognostic, therapeutic or blood banking) is unknown.


Product Details

9
A simple and rapid plate-based workflow
Partitioning, thermocycling and imaging are all integrated into a single fully automated instrument, delivering results in under two hours. Nanoplate formats are amenable to front-end automation for sample preparation, further reducing hands-on time.
Performance

The QIAcuity Digital PCR System makes absolute quantification accessible and affordable for all laboratories. The walk-away automation integrates and streamlines the entire digital PCR workflow of partitioning, thermocycling and imaging into a single instrument with minimal hands-on time. It is also easy to adapt your current qPCR assays to the QIAcuity Digital PCR System. No change in plate handling is required when coming from qPCR, assuring fast assay setup and quick results in under 2 hours.

QIAcuity Instruments – features and specifications

Feature QIAcuity One QIAcuity Four QIAcuity Eight
Plates processed 1 4 8
Detection channels (multiplexing) 2 or 5 5 5
Thermocycler(s) 1 1 2
Time to result Under 2 h

First plate under 2 h

Every ~60 min a following plate

First plate under 2 h

Every ~30 min a following plate

Throughput (samples processed in a work day)

Up to 384 (96-well)

Up to 96 (24-well)

Up to 672 (96-well)

Up to 168 (24-well)

Up to 1248 (96-well)

Up to 312 (24-well)

 

Principle

In just 3 simple steps, you can have the dPCR result you want in under 2 hours: pipette and load, run the experiment, analyze results.

The principle of the dPCR reaction in the nanoplates is described here.

Procedure

Just like in qPCR experiments, sample preparation includes the transfer of master mix, probes and primers to a 96- or 24-well nanoplate, followed by the addition of samples. The system integrates partitioning, thermocycling and imaging into a single fully automated instrument that takes users from the sample to result in under 2 hours. One can perform analysis on the Software Suite, providing the concentration in copies per microliter of your target sequence as well as for quality control such as positive samples or NTC. This analysis can also be extended to remote computers within the same local area network (LAN).

Applications

The QIAcuity instruments, together with the QIAcuity Nanoplates and the QIAcuity PCR kits, enable digital PCR applications, including:

  • Rare mutation detection
  • Copy number variation analysis
  • Gene expression analysis
  • Pathogen detection
  • Genotyping
  • miRNA research
Software

The QIAcuity Software Suite provided with the instrument and installed on a separate computer controls one or multiple QIAcuity instruments, either connected directly to one instrument or using an existing local area network (LAN). Using the QIAcuity Software Suite, digital PCR experiments, samples and reaction mixes can be defined, assigned to Nanoplates, and transferred to the QIAcuity instrument. After the run, data can be analyzed, reports can be created and data can be exported for external analysis. The software provides multiple template functionalities to make repeating plate layouts or plate run parameters easily accessible, further transforming your digital PCR experience.

When integrated into a local area network, the computer is hosting the QIAcuity Software Suite function as a server that is accessible via LAN to other computers serving as clients. This enables multiple users to access the software from other rooms or offices and analyze data via a standard browser without the need to install the software on multiple computers or access and exchange data via internet connections.

Services

Protect your instrument with diverse service solutions from QIAGEN. Learn more about a specific service agreement to match your needs.

Service Plans

Cat No./ID: 9245357
QIAcuity Eight Preventive Subscription

One on-site preventive maintenance (PM), including travel, labor, and PM parts. Includes a 10% discount on repair services during Preventive Subscription period and documentation in a GMP/GLP-compliant visit report.

Cat No./ID: 9245359
QIAcuity One 5plex Core Agreement

One on-site repair service and one-on-site preventive maintenance including travel, labor, and spare parts for a period of one year. Response time of five business days. Includes a 10% discount on additional repair service during agreement period.

Cat No./ID: 9245356
QIAcuity Four Preventive Subscription

One on-site preventive maintenance (PM), including travel, labor, and PM parts. Includes a 10% discount on repair services during Preventive Subscription period and documentation in a GMP/GLP-compliant visit report.

Cat No./ID: 9245355
QIAcuity One Preventive Subscription

One on-site preventive maintenance (PM), including travel, labor, and PM parts. Includes a 10% discount on repair services during Preventive Subscription period and documentation in a GMP/GLP-compliant visit report.

Cat No./ID: 9245353
QIAcuity Four Installation & Training

Installation of instrument hardware and system software. Includes application training for hands-on experience in operating the instrument and qualifying laboratory staff. Labor, travel, and installation kit (if applicable) included.

Cat No./ID: 9245358
QIAcuity One 2plex Core Agreement

One on-site repair service and one-on-site preventive maintenance including travel, labor, and spare parts for a period of one year. Response time of five business days. Includes a 10% discount on additional repair service during agreement period.

Cat No./ID: 9245352
QIAcuity One Installation & Training

Installation of instrument hardware and system software. Includes application training for hands-on experience in operating the instrument and qualifying laboratory staff. Labor, travel, and installation kit (if applicable) included.

Cat No./ID: 9245354
QIAcuity Eight Installation & Training

This service combines Installation with Applications Training to help customers gain hands-on experience of hardware, software, applications using QIAGEN platforms and facilitates professional qualifications of laboratory staff.

Cat No./ID: 9245362
QIAcuity One 2plex Full Agreement

On-site QIAcuity One 2plex instrument repair, including travel, labor, and parts, for a period of one year. Response time of two business days. Includes one preventive maintenance during the Full Agreement period.

Cat No./ID: 9245364
QIAcuity Four Full Agreement

On-site QIAcuity Four instrument repair, including travel, labor, and parts, for a period of one year. Response time of two business days. Includes one preventive maintenance during the Full Agreement period.

Cat No./ID: 9245360
QIAcuity Four Core Agreement

One on-site repair service and one-on-site preventive maintenance including travel, labor, and spare parts for a period of one year. Response time of five business days. Includes a 10% discount on additional repair service during agreement period.

Cat No./ID: 9245365
QIAcuity Eight Full Agreement

On-site QIAcuity Eight instrument repair, including travel, labor, and parts, for a period of one year. Response time of two business days. Includes one preventive maintenance during the Full Agreement period.

Cat No./ID: 9245361
QIAcuity Eight Core Agreement

One on-site repair service and one-on-site preventive maintenance including travel, labor, and spare parts for a period of one year. Response time of five business days. Includes a 10% discount on additional repair service during agreement period.

Cat No./ID: 9245363
QIAcuity One 5plex Full Agreement

On-site QIAcuity One 5plex instrument repair, including travel, labor, and parts, for a period of one year. Response time of two business days. Includes one preventive maintenance during the Full Agreement period.

The QIAcuity is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease. Therefore, the performance characteristics of the product for clinical use (i.e., diagnostic, prognostic, therapeutic or blood banking) is unknown.


Product Resources

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Operating Software (6)
Version 1.0

The Volume Precision Factor (VPF) offers a unique feature to secure precision of concentration results obtained from a QIAcuity dPCR run. 
In general, Nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. Potential variation of partition sizes in Nanoplate batches, caused by different microstructure molding forms, can be addressed by applying the batch specific VPF. Furthermore, the VPF includes well-specific volume information and therefore further increases precision of concentration calculation in each well of the Nanoplates.

After downloading and updating the VPF file within the QIAcuity Software Suite, the VPF is applied automatically to the analysis of a corresponding Nanoplate batch. The VPF file includes information from all available microstructure molding forms and connected Nanoplate batches. It will be stored on the PC where the QIAcuity Software Suite is installed. 

Required QIAcuity Software Suite version: Version 1.2 or higher.
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Version 1.1.3
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Version 1.0

The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis.

This new version of the control software includes system stability and performance reliability improvements. We highly recommend to update the control software of your QIAcuity instrument.

Note: The installation requires approximately 90 minutes.
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For Version 1.0
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For Version 1.2
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Version 1.2

The QIAcuity Software Suite 1.2 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).

The following browsers are supported in the QIAcuity Software Suite:

-Mozilla Firefox (version 64.0.2 or higher)
-Microsoft Edge (version 44.17763.1.0 or higher)
-Google Chrome (version 71.0.3578.98 or higher)

The new QIAcuity Software Suite 1.2 offers a functionality that enables users of the QIAcuity Software 1.1.3 to upgrade to the new version while keeping the library of previously stored plate runs.

Note: If you have exported plates from QIAcuity Software Suite 1.1.3 that you would like to import and use in QIAcuity Software Suite 1.2, you will need to import the plates before upgrading from version 1.1.3 to version 1.2. You may then export the plates again. Future software version starting from QIAcuity Software Suite 2.0 will facilitate import of plates from previous QIAcuity Software Suite versions.

The new improvements are as follows:

-Support for the Nanoplate 8.5k 24-well
-Hyperwell functionality to combine several wells to one combined well for analysis
-Automated plate archiving functionality
-Functionality to show the number of single/double positives in 2D scatterplots
-VPF (Volume Precision Factor) to further improve concentration calculation (see related resources)
-Additional improvements for stabilization and troubleshooting


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Instrument User Manuals (5)
User manual for QIAcuity instrument
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For use with QIAcuity systems
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Network connection of QIAcuity instruments
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For QIAcuity Software Suite 1.2: Updating VPF for archived or exported plates
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