RNA QC PCR Arrays

RNA QC PCR Arrays are designed to assess the quality of chicken, Chinese hamster, cow, dog, fruit fly, horse, human, mouse, pig, rabbit, rat, Rhesus macaque or zebrafish RNA samples before characterization with a cataloged or custom RT² Profiler PCR Array. The arrays contain a number of PCR controls that test for RNA integrity, inhibitors of reverse transcription and PCR amplification, and genomic and general DNA contamination.

RNA QC PCR Arrays (for chicken, Chinese hamster, cow, dog, fruit fly, horse, human, mouse, pig, rabbit, rat, Rhesus macaque or zebrafish) are designed to assess the quality of RNA samples before characterization with cataloged or custom RT² Profiler PCR Arrays. The arrays contain a number of PCR controls for RNA integrity, inhibitors of reverse transcription and PCR amplification, genomic and general DNA contamination (see below). Failure of any of these controls indicates that the RNA could lead to false negative or false positive results in SYBR^® Green-based real-time PCR.

RT² RNA QC PCR Arrays offer added confidence in real-time PCR results. They are especially useful for ensuring the consistency of a large number of samples across an entire study by performing RNA quality control on up to 12 samples on one plate simultaneously. Using an RT² RNA QC PCR Array to test the quality of RNA samples avoids potentially wasting mastermix and PCR arrays on substandard samples.

Controls

The RT² RNA QC PCR Array includes controls for:

• RNA integrity: Two housekeeping genes (HK1, HK2)
• Genomic DNA contamination: Genomic DNA control (GDC)
• Genomic DNA contamination: No reverse transcription control (NRT)
• General nonspecific DNA contamination: No-template control (NTC)
• Reverse transcription inhibition: Reverse transcription control (RTC)
• PCR amplification inhibition: Positive PCR control (PPC)

An RT² First Strand Kit must be used to synthesize cDNA template from total RNA in order to accurately interpret all control elements on the RT² RNA QC PCR Array.

Formats

The human, mouse, rat, dog, and Rhesus macaque RT² RNA QC PCR Arrays are available in the following formats.

• RT² RNA QC PCR Array Format A: 96-well RT² RNA QC PCR Array, 12 Optical Thin-Wall 8-Cap Strips
• RT² RNA QC PCR Array Format C: 96-well RT² RNA QC PCR Array, Optical Adhesive Film
• RT² RNA QC PCR Array Format D: 96-well RT² RNA QC PCR Array, 12 Optical Thin-Wall 8-Cap Strips
• RT² RNA QC PCR Array Format E: 384-well RT² RNA QC PCR Array, Optical Adhesive Film, 384EZLoad Covers
• RT² RNA QC PCR Array Format F: 96-well RT² RNA QC PCR Array, Optical Adhesive Film
• RT² RNA QC PCR Array Format G: 384-well RT² RNA QC PCR Array, Optical Adhesive Film, 384EZLoad Covers
• RT² RNA QC PCR Array Format R: Rotor-Disc 100 RT² RNA QC PCR Array, Rotor-Disc Heat Sealing Film
  

Each cDNA template (generated using a RT² First Strand Kit) is mixed with the PCR master mix and equal volumes of each aliquotted to the HK1, HK2, GDC, RTC, and one of the two PPC wells of each sample’s column. Each RNA sample is mixed with PCR master mix and added to the samples’ NRT well. Diluted PCR master mix is added to the NTC and second PPC wells before the real-time PCR cycling program is run.

Chicken, Chinese hamster, cow, dog, fruit fly, horse, human, mouse, pig, rabbit, rat, Rhesus macaque or zebrafish RT² RNA QC PCR Arrays are highly suited to assess the quality of RNA samples before characterization using an RT² Profiler PCR Array in real-time PCR-based gene expression analysis.