QIAamp 96 Viral RNA Kit
For high-throughput purification of viral RNA
The QIAamp 96 Viral RNA Kit enables simple, purification of viral RNA manually with a plate centrifuge. Various options for automation on instruments including the Fluent 780 from Tecan, or the epMotion 5075V from Eppendorf support high-throughput processing with minimal hands-on time. Using proven QIAamp silica-membrane technology in a convenient 96-well format, contaminants and inhibitors are removed to yield high-quality nucleic acids directly ready for downstream analysis.
The QIAamp 96 Viral RNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
The QIAamp 96 Viral RNA Kit has been successfully used to recover RNA from viruses, including SARS-CoV-2 virus (Comparison of SARS-CoV-2 isolation). Performance of the QIAamp 96 Viral RNA Kit is comparable to that achieved with the QIAamp Viral RNA Mini Kit (Reliable RNA recovery). RNA isolation is proven from commonly used saliva collection and stabilization methods (Compatability with saliva stabilization methods) and no cross-contamination was detected (No cross-contamination).
The QIAamp 96 Viral RNA Kit simplifies isolation of viral RNA from nasotracheal swabs. No phenol–chloroform extraction is required. Viral RNA binds specifically to the QIAamp silica membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure viral RNA to be eluted in either water or a buffer provided with the kit.
Optimized buffers and enzymes lyse samples, stabilize nucleic acids, and enhance selective RNA adsorption to the QIAamp membrane. To guarantee RNA integrity, samples are lysed under highly denaturing conditions to inactivate RNases. Alcohol is added and lysates loaded onto the QIAamp 96 Plate. Wash buffers are used to remove impurities and pure, ready-to-use RNA is then eluted in water or low-salt buffer. The QIAamp 96 Viral RNA Kit is automatable on various instruments.
We recommend use of the Centrifuge 4-16S or Centrifuge 4-16KS for centrifuge processing. Alternative centrifuges with swing-out rotor, minimum 5788 x g capacity, and the ability to accommodate plate assemblies with a height of minimum 75 mm or more may be used. Centrifuges with lower g-force (minimum 3486 x g) can be used with an alternative protocol.
Plasticware recommended for centrifugation processing: Plastic consumables need to be in SBS format and must be able to withstand the necessary g-forces. Deep-well plates need to have a capacity of 2 ml and elution plates need to have a capacity of ≥500 µl. Four deep-well plates and one elution plate are required for processing of 96 samples.
The QIAamp 96 Viral RNA Kit simplifies high-throughput viral RNA, offering fast 96-well procedures, or automation on suitable centrifuges and instruments.
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