PCR酵素とキット
高特異的かつ高感度のPCR
遺伝子増幅を行う際に、実験の成功はスループット、アッセイ感度、テンプレートの難易度に起因しない反応のロバストネス、データ正規化の信頼性など、いくつかの重要な要素に左右される可能性があります。GC含有量が多く、テンプレート量が少なく、反応状態の最適化が必要な難しいテンプレートは、新たな課題が生じます。
当社のPCR試薬およびPCR酵素によって、成功に導き、こうした課題を克服しやすくなります。PCRキットのデュアルカチオンPCRバッファーは、幅広い温度にわたり高いプライマーアニーリング特異性を維持するため、個々のプライマーテンプレートシステムごとにPCRを最適化しなくて済みます。
Product category | Your application goal | Recommended products | When to choose |
|---|---|---|---|
| Hot start PCR |
| For robust, reliable amplification of standard targets | |
| Multiplex PCR | Amplify multiple DNA targets in one reaction | When detecting several targets simultaneously and reducing sample input | |
| One-step RT-PCR | Translate RNA target into DNA in one reaction (RT + PCR) | When starting from RNA and no separate cDNA storage is required | |
| Reverse transcription | Convert RNA into cDNA for downstream applications | When flexible downstream assays or long-term storage of cDNA is needed | |
| High fidelity & long range PCR | High-fidelity amplification for sequencing or cloning | When sequence accuracy or long amplicons (>5–10 kb) are required | |
| PCR enzymes (bulk & OEM) | Custom assay development or OEM supply | For specific or industrial applications and custom formulations |
Find the right end-point PCR product for your workflow
Once you’ve identified the right solution for your application, use the table below to explore detailed specifications of individual PCR kits and enzymes.
It provides key technical parameters such as template type, amplicon length, fidelity, multiplex capability, and performance characteristics — helping you select the most suitable product for your specific workflow.
Product | Template | Amplicon length | Exonuclease activity | Resulting ends | Proof-reading | Multiplex capability | Hot start | Extension time | NGS-ready | GC-Rich or difficult templates |
|---|---|---|---|---|---|---|---|---|---|---|
| Hot Start PCR HotStarTaq DNA Polymerase | DNA | 3–4 kb | Yes 5'→3' | 3' dA overhang | No | No | Yes Chemically-mediated | ∼1 kb/min | No | Yes With Q-Solution |
| Hot Start PCR AllTaq PCR Core Kit | DNA | Up to 9 kb | Yes 5'→3' | 3' dA overhang | No | Yes 2 targets | Yes Antibody-mediated | ∼1 kb/min | No | Yes With Q-Solution |
| Multiplex PCR QIAGEN Multiplex PCR Kit | DNA | ∼2–3 kb | Yes 5'→3' | 3' dA overhang | No | Yes Up to 16 targets | Yes Antibody-mediated | ∼1 kb/min | No | Yes With Q-Solution |
| Multiplex PCR UCP Multiplex PCR Kit | DNA | ∼3–4 kb | Yes 5'→3' | 3' dA overhang | No | Yes Up to 22 targets | Yes Antibody-mediated | ∼1 kb/min | No | Yes With Q-Solution |
| One-step RT-PCR QIAGEN OneStep RT-PCR Kit | RNA | ≤ 2 kb | Yes 5'→3' | 3' dA overhang | No | No | Yes Chemically-mediated | ∼1 kb/min | No | Yes With Q-Solution |
| One-step RT-PCR QIAGEN OneStep Ahead RT-PCR | RNA | ≤ 4 kb | Yes 5'→3' & 3'→5' | Mixture, 3′ dA overhang and blunt ends | Yes | Yes 2 targets | Yes Antibody-mediated | Fast cycling (∼60 min protocol) | No | Yes With Q-Solution |
| Reverse transcription Omniscript RT Kit | RNA | 1–3 kb | N/A | N/A | N/A | No | No | N/A | No | Yes |
| Reverse transcription EnzScript Reverse Transcriptase | RNA | cDNA transcripts >9.4 kb | N/A | N/A | N/A | No | No T° tolerance up to 50°C | N/A | Yes library construction for RNA-Seq transcriptome studies | No |
| High fidelity & long range PCR UltraRun LongRange PCR Kit | DNA | Up to 30 kb | Yes 3'→5' | Blunt ends | Yes [+dU tolerant] | Yes Up to 6 targets | Yes Antibody-mediated | ~1kb / 30s | No | Yes With Q-Solution |
| High fidelity & long range PCR 2x Hi-Fi PCR Master Mix | DNA | Up to 4 kb | Yes 3'→5' | Blunt ends | Yes | Yes library amplification | Yes Antibody-mediated | ~1kb / 30s | Yes NGS library amplification | Yes |
| High fidelity & long range PCR VeraSeq PCR Mix | DNA | Up to 8 kb | Yes 3'→5' | Blunt ends | Yes | Yes Can used for higher order amplicon pools for sequencing | No | ~1kb / 15s | No | No |
| High fidelity & long range PCR VeraSeq 2.0 High-Fidelity DNA Polymerase | DNA | Up to 8 kb | Yes 3'→5' | Blunt ends | Yes | Yes Can be used for higher order amplicon pools for sequencing | No | ~1kb / 15s | No | Yes with 5X VeraSeq GC buffer |
| High fidelity & long range PCR VeraSeq 2.0 Ultra DNA Polymerase | DNA | Up to 8 kb | Yes 3'→5' | Blunt ends | Yes [+dU tolerant] | Yes Can be used for higher order amplicon pools for sequencing | No | ~1 kb / 15s | No | Yes with 5X VeraSeq GC buffer |
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21個の製品が見つかりました
VeraSeq™ ULtra DNA Polymerase
Ultrathermostable uracil-literate polymerase for high-fidelity PCR
VeraSeq™ 2.0 High-Fidelity DNA Polymerase
Ultrathermostable polymerase for high-fidelity PCR
2x HiFi PCR Master Mix
A high efficiency, high-fidelity and low bias PCR master mix for NGS library amplification.
VeraSeq™ PCR Mix
Ultrathermostable mastermix for high-fidelity PCR
QIAGEN OneStep RT-PCR Kit
高感度で特異的な1ステップRT-PCR
QIAGEN Multiplex PCR Kit
至適化なしに特異性および感度の高いマルチプレックスPCR
HotStarTaq DNA Polymerase
最小限の至適化で特異性の高い増幅反応
HotStarTaq Master Mix Kit
どのようなPCRアプリケーションでも特異性の高い増幅を実現
QIAGEN Multiplex PCR Plus Kit
高度なアプリケーションで良好な結果を簡便に実現するマルチプレックスPCR
AllTaq Master Mix and PCR Core Kits
あらゆるアプリケーションで高速かつ多彩なホットスタートPCR
TaqDNA Polymerase
標準的および特殊なPCR 用
Type-it Microsatellite PCR Kit
マイクロサテライト遺伝子座の迅速で確実なマルチプレックスPCR解析
Omniscript RT Kit
反応あたり50 ng ～ 2 μg のRNA を用いた逆転写反応（エンドポイントPCR用）
Sensiscript RT Kit
反応あたり50 ng 未満のRNA を用いた逆転写反応
HotStarTaq Plus DNA Polymerase
すべてのアプリケーションで迅速かつ特異的な増幅
Taq PCR Master Mix Kit
簡便な PCR セットアップ用マスターミックス
HotStarTaq Plus Master Mix Kit
すべてのアプリケーションで迅速かつ特異的な増幅
UltraRun LongRange PCR Kit
For ultrafast, hot-start mediated, long-range PCR
QIAGEN OneStep Ahead RT-PCR Kit
感度・特異性が高く信頼のおける、高速1ステップRT-PCR 用キット
UCP Multiplex PCR Kit
Ultra-Clean Production master mix for multiplex hot-start PCR and microbiome applications
Type-it Mutation Detect PCR Kit
マルチプレックスPCRにより変異を高い再現性で正確に解析
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