QIAamp DNA Blood BioRobot 9604 Kit
For automated purification of genomic and mitochondrial DNA from whole blood using the BioRobot 9604 workstation
The QIAamp DNA Blood BioRobot 9604 Kit provides automated DNA purification on the BioRobot 9604 workstation using proven QIAamp silica-membrane technology. The fully automated procedure on the BioRobot 9604 workstation requires less than 2.5 hours, including bar code reading and complete process documentation, with no hands-on time during the run.
QIAamp 96-well plates provide well-to-well uniformity in DNA recovery and purity (see figures "Reproducible DNA yield" and "Reproducible DNA purity"), and PCR experiments show no cross-contamination between samples in adjacent wells (see figure "Cross-contamination-free purification for reliable PCR"). As many as 96 samples of human whole blood or other body fluids can be processed yielding purified DNA in under 2 hours with minimal hands-on time. QIAamp sample preparation technology is fully licensed.
The QIAamp 96 DNA Blood procedure on the BioRobot 9604 increases productivity and reliability. DNA purified using the QIAamp 96 DNA Blood procedure on the BioRobot 9604 is free of contaminants and enzyme inhibitors, and is ready for use in amplification, Southern blotting, or other enzymatic assays for
No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in three efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp technology yields genomic and mitochondrial DNA from blood and other body fluids ready to use in PCR and blotting procedures.
Fresh and frozen whole blood with common anticoagulants, such as citrate, EDTA, and heparin, may be processed with additional equipment and the use of a special protocol available from QIAGEN Technical Services or your local distributor.
The QIAamp DNA Blood BioRobot 9604 Kit simplifies isolation of DNA from blood and other body fluids with a fast automated 96-well–plate procedure. Using the BioRobot 9604 Workstation, the QIAamp DNA Blood BioRobot 9604 Kit processes up to 200 µl sample size, with preparation time of 96 samples in less than 2 hours (see "Protocol"). The automated procedure, including bar code reading and process documentation, needs about 5 minutes hands-on time, with a turnaround time between consecutive runs of about 10 minutes. The typical yield is 3–6 µg per 200 µl healthy whole blood, with an elution volume of 200 µl.
The QIAamp DNA Blood BioRobot 9604 Kit provides automated high-throughput DNA purification on the BioRobot 9604 using proven QIAamp technology. The kit processes 96-well plates for up to 96 preps. Sample types include:
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