PAXgene Blood DNA Tubes

在使用PAXgene Blood DNA Kit纯化基因组DNA前,用于血液收集

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PAXgene Blood DNA Tubes (100)

Cat. No. / ID:  761115

100 PAXgene Blood DNA Tubes
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US$201.00
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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 室温下存储14天以上
  • 易于操作
  • 在同一体系中进行血液收集及纯化
  • 提高工作流程的效率

Product Details

PAXgene Blood DNA系统包括用于血液采集和稳定的PAXgene Blood DNA Tubes和用于单管纯化DNA的PAXgene Blood DNA Kit。

Performance

PAXgene Blood DNA System分离的DNA A260/A280的比值在1.7–1.9。片段大小20–200 kb,平均长度50–150 kb(参见" High quality and high molecular weight of genomic DNA")。DNA平均产量为150–500 µg,由血样中的核细胞数目决定。纯化的DNA可用于PCR、定量和real-time PCR(参见" Efficient multiplex PCR of 3 mitochondrial genes")。
See figures

Principle

PAXgene Blood DNA System是整合的标准化体系,用于收集和稳定全血样本并分离基因组DNA。该体系通过PAXgene Blood DNA Tubes收集和稳定血液,并使用PAXgene Blood DNA Kit纯化DNA。

PAXgene Blood DNA Tubes包含可防止血液凝结以及可稳定白细胞的混合试剂,用来收集血液。血液样本可在室温下在管中储存长达14天。用PAXgene Blood DNA Kit中提供的缓冲液分离DNA。获得的高纯度的基因组DNA适合各种下游应用。

Procedure

PAXgene Blood DNA操作步骤如流程图" PAXgene Blood DNA procedure"所示。血液样本(8.5 ml)由PAXgene Blood DNA Tubes收集,并储存或运输。如需分离DNA,则将血液转入处理管中(已预充细胞裂解缓冲液),将溶液混合,裂解红细胞和白细胞。细胞核和线粒体通过离心收集、洗涤,重悬于裂解缓冲液。蛋白污染物经蛋白酶孵育后去除。DNA用异丙酮沉淀、70%乙醇洗涤、干燥,并重悬于缓冲液。

See figures

Applications

纯化的DNA可用于各种下游应用,包括:

  • PCR和定量real-time PCR
  • Southern印迹
  • SNP基因分型
  • 药物基因组研究
  • SNP发现和鉴定

Supporting data and figures

Specifications

FeaturesSpecifications
Tube size16 x 100 mm
Draw volume8.5 ml
Label typePaper
Quantity8.5 ml
Closure type/colorBD Hemogard/Blue
Additive/concentrationAdditive 2 ml

Resources

产品介绍与指南 (1)
For collection, storage, and transport of whole blood for genomic DNA isolation

 

安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
试剂盒操作手册 (3)
For the collection of whole blood for genomic DNA isolation

 

Safety Data Sheets (1)
Certificates of Analysis (1)
Brochures & Guides (1)
For collection, storage, and transport of whole blood for genomic DNA isolation

 

Kit Handbooks (3)
For the collection of whole blood for genomic DNA isolation

 

FAQ

Where can I find additional information for PreAnalytiX PAXgene products?
You can find additional information relating to the PreAnalytiX PAXgene products on the PreAnalytiX website .
FAQ ID - 3515
Does the DNA extracted using the PAXgene Blood DNA System contain RNA?

No. DNA purified with the PAXgene Blood DNA System is free of detectable RNA contamination. In contrast to commercial salting out methods, additional RNase digestion steps are not necessary.

FAQ ID - 3499
How should blood samples drawn into PAXgene Blood DNA Tubes be transported?

See subsequent FAQs for information about storage interval and temperature. Each laboratory should validate shipping procedures.

FAQ ID - 3507
How long can samples in PAXgene Blood DNA Tubes be stored at room temperature?

Samples in PAXgene Blood DNA Tubes can be stored at room temperature (18–25°C) for up to 14 days after blood collection.

FAQ ID - 3508
How long can the PAXgene Blood DNA Tube be kept at room temperature? What is room temperature?

The PAXgene Blood DNA Tubes can be kept at room temperature for up to 14 days after blood collection. Room temperature is designated as 18–25°C.

FAQ ID - 3492
How should I thaw frozen PAXgene Blood DNA Tubes?

Thaw the PAXgene Blood DNA Tubes in a wire rack at ambient temperature (18–25ºC) for approximately two hours or at up to 37°C in a water bath for approximately 15 minutes. Carefully invert the thawed PAXgene Blood DNA Tubes 10 times.

FAQ ID - 3495
How should blood samples drawn into the PAXgene Blood DNA Tubes be stored?

Blood samples in PAXgene Blood DNA Tubes can be stored at room temperature (18–25°C) for up to 14 days or at 2–8°C for up to 28 days. For long-term storage, samples should be frozen at –20°C.

FAQ ID - 3490
3497 - What is the expected purity and size of DNA extracted using the PAXgene Blood DNA kit?

The typical A260/A280 ratio is from 1.7 to 1.9. The DNA molecules are up to 200 kb in size, with fragments of 50–150 kb predominating.

FAQ ID - 3497
Do I have to use a blood collection set with the PAXgene Blood DNA Tube?

No, a blood collection set is not required to use PAXgene Blood DNA Tubes. Further information on phlebotomy technique with PAXgene Blood DNA Tubes IVD can be found in the product handbook.

FAQ ID - 3484
Can the PAXgene Blood DNA Tubes be stored at 4°C?

Yes, the PAXgene Blood DNA Tubes can be stored at 4°C for up to 28 days after blood collection.

FAQ ID - 3493
Can PAXgene Blood DNA Tubes be stored at higher than room temperature (18–25°C)?

Yes, samples in PAXgene Blood DNA Tubes can be stored at 30°C for up to 3 days after blood collection. However, it is not recommended to store blood samples at temperatures higher than 30°C and storage at high temperatures should not exceed 3 days.

FAQ ID - 3509
What is the shelf life of the PAXgene Blood DNA Tube prior to blood collection?

The shelf life of unused PAXgene Blood DNA Tubes is 1 year. The expiration date is printed on the tube label and on the shelf carton. Store PAXgene Blood DNA Tubes at room temperature (18–25°C).

FAQ ID - 3488
What is the expected purity of DNA extracted from blood samples collected in PAXgene Blood DNA Tubes IVD?

The typical A260/A280 ratio is from 1.7 to 1.9.

FAQ ID - 3511
Can the PAXgene Blood DNA Tube be frozen? If yes, at what temperatures and for how long?

The PAXgene Blood DNA Tubes can be stored at –20ºC for up to 3 months. If tubes are to be kept at temperatures below –20ºC, freeze them first at –20ºC for 24 hours, then transfer them to –70ºC or –80ºC.
To freeze PAXgene Blood DNA Tubes, stand them upright in a wire rack or horizontally in a plastic bag. Do not freeze tubes upright in a styrofoam tray as this may cause the tubes to crack.

FAQ ID - 3491
What is the expected yield from 8.5 ml of blood?

Yield of genomic DNA will vary depending on the cell count from the donor blood sample but average range is from 150 µg to 500 µg.

FAQ ID - 3496
Can mitochondrial DNA be isolated with the PAXgene Blood DNA System?

Yes, mitochondrial and genomic DNA are both isolated.

FAQ ID - 3498
Can smaller volumes of blood be processed from the PAXgene Blood DNA Tube?

No. The volume of Buffer BG1 (lysis buffer) prefilled in the processing tubes, and all the other buffer volumes are optimized for use with blood from one PAXgene Blood DNA Tube.

FAQ ID - 3500
Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes IVD?

To date, DNA purified from blood samples in PAXgene Blood DNA Tubes has been successfully tested for use in PCR, including multiplex PCR.

FAQ ID - 3512
2. Are there any special considerations for the collection of blood using PAXgene Blood DNA Tubes CE (e.g., use of a blood collection set or discard tube, positioning of the tube, issues with short draws, etc.)?

Blood collection using PAXgene Blood DNA Tubes is performed using standard phlebotomy protocol. Blood is collected into the evacuated tube using a needle or blood collection set with a standard needle holder that can accommodate a 13 mm diameter tube. Further information on phlebotomy technique with PAXgene Blood DNA Tubes can be found in the product handbook.

FAQ ID - 3485
How should DNA purified using the PAXgene Blood DNA System be stored?

DNA purified using the PAXgene Blood DNA System should be dissolved in Buffer BG4 and stored at 2–8°C. For long-term storage, DNA can be stored at –20°C but multiple freezing and thawing cycles should be avoided.

FAQ ID - 3502
What is the additive in PAXgene Blood DNA Tubes

PAXgene Blood DNA Tubes contain a proprietary EDTA formulation. Contents of the tube may cause irritation to eyes, respiratory system, and skin. For more information, please refer to the product handbook, download the MSDS, or contact BD Technical Service.

FAQ ID - 3486
What downstream applications have been tested with DNA purified using the PAXgene Blood DNA System?

So far, we have shown that DNA purified using the PAXgene Blood DNA System can be used in PCR, including long range and multiplex PCR, restriction digestion and Southern analysis, as well as in various SNP detection procedures including,TaqMan®, LightCycler and Masscode technologies.

See trademarks

FAQ ID - 3506
Can the PAXgene Blood DNA Tubes be stored at higher temperatures?
Yes, the PAXgene Blood DNA Tubes can be stored at 30°C for up to 7 days after blood collection. However it is not recommended to store blood sample at temperatures higher than 30°C
FAQ ID - 3494
Can I use the PAXgene Blood DNA Tube to collect blood from animals?

The system is designed for use only with human blood samples.

FAQ ID - 3504
How should blood samples drawn into PAXgene Blood DNA Tubes be shipped?

See subsequent FAQs for specific storage temperature and time information. Individual laboratories should validate shipping procedures.

FAQ ID - 3489
Are the PAXgene Blood DNA Tubes sterile?

The inside of the PAXgene Blood DNA Tube is sterilized during production.

FAQ ID - 3487