RT2 qPCR Primer gDNA Controls



RT2 qPCR Primer gDNA Controls 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。
RT2 qPCR Primer gDNA Control (200)

Cat. No. / ID:  330011

RT2 qPCR Primer gDNA Contam. Control
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  • 灵敏检测非转录的基因组DNA
  • 适用于多个物种
  • 可与RT² qPCR Primer Assays配套使用

Product Details

RT2 qPCR Primer gDNA Controls专用于检测非转录的基因组DNA,灵敏度高。RT2 qPCR Primer gDNA Controls适用于人类、小鼠、大鼠、狗和猕猴等生物。每瓶含有足够200次PCR反应的引物。


RT² qPCR Primer gDNA Controls专用于检测非转录的人类、小鼠、大鼠、狗或恒河猴的基因组DNA,灵敏度高。在进行SYBR® Green实时荧光定量PCR之前,此产品可帮助检测cDNA样本中的基因组DNA污染,避免假阳性信号。含有基因组DNA污染的RNA样本通过合适的不含RNAse的DNAse的处理,吸附柱纯化,然后再用RT² qPCR Primer gDNA Controls检测。不含有基因组DNA污染的样本可在基因表达分析中获得准确的结果。


安全数据表 (1)
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仪器技术参数 (1)
For pathway-focused gene expression analysis
Safety Data Sheets (1)
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How important is the RNA purification process, for obtaining reliable qRT-PCR results?

The most important prerequisite for any gene expression analysis experiment is the preparation of consistently high-quality RNA from every experimental sample. Contamination by DNA, protein, polysaccharide, or organic solvents can jeopardize the success of an experiment.

Genomic DNA contamination in an RNA sample compromises the quality of gene expression analysis results. The contaminating DNA inflates the OD reading of the RNA concentration. It is also a source of false positive signals in RT-PCR experiments.

RNase contamination degrades RNA samples whichcauses low signal and false-negative results in PCR.

Residual polysaccharides, collagen, other macromolecules, and organic solvents in an RNA sample can inhibit the activity of DNase, which may interfere with DNase treatment for genomic DNA removal. These contaminants may also inhibit reverse transcriptase and DNA polymerase, leading to lower reverse transcription efficiency and reduced PCR sensitivity.

For fast purification of high-quality RNA we recommend QIAGEN’s RNeasy Kits like the RNeasy Mini Kit, the RNeasy Plus Universal Kit, or the RNeasy FFPE Kit.

FAQ ID -2655