Products
RT2 lncRNA PCR Array Modification 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。
Features
- 较为经济地将您所感兴趣的lncRN替换到现有信号转导或疾病通路中
- 可选择任意现有的RT2 lncRNA qPCR Assay引物或RT2 qPCR Primer Assay引物
- 所有检测引物的扩增效率、特异性及灵敏度均经过湿法验证
- 在保留专利质控孔的基础上可替换最多4个基因或lncRNA
Product Details
RT2 lncRNA PCR Array Modification结合了RT2 lncRNA PCR Array中精选过的lncRNA，以及至多四个其他您所感兴趣的lncRNA。您可以从现有列表中去除任意4个assay并用自己选择的lncRNA assay进行替代，具体的lncRNA assay可从人或小鼠的RT2 lncRNA qPCR Assay列表中选择。
Performance
Sensitivity
The sensitivity of the RT2 First Strand Kit enables gene expression analysis using as little as 1 ng of total RNA. With preamplification, more of your targets can be accurately quantitated using the complete PCR array system.
Reproducibility
The complete PCR array system demonstrates strong correlations across technical replicates, lots and instruments with average correlation coefficients >0.99, ensuring reliable detection of differences in expression between biological samples.
Specificity
The PCR array system, with high-quality input RNA, yields single bands of the predicted size without primer-dimers or other secondary products, therefore providing highly accurate real-time PCR results.
Uniform PCR amplification efficiency
Uniform PCR amplification efficiency is required for the PCR array technology to allow accurate comparisons of gene expression across all genes and all samples. The unique combination of our proprietary primer design algorithm and rigorous testing of every primer assay guarantees the high performance of every primer assay on PCR arrays.
A guaranteed system
RT2 Profiler PCR Arrays are tested and optimized in combination with the RT2 SYBR® Green qPCR Mastermixes and the RT2 First Strand Kit. This testing means that RT2 Profiler PCR Array performance is guaranteed when all three of these components are used together.
Principle
RT2 lncRNA PCR Array是一组RT2 lncRNA qPCR Assay引物产品的组合，专门用于检测信号转导或疾病相关的多个长链非编码RNA。这些引物通过一个特定的算法设计而成，并结合相关体外分析方法，可确保所设计的引物序列在确保引物的扩增效率达到100 +/– 10%的情况下能够在33个PCR扩增循环内生成与预期产物大小相同的单一PCR产物，而不会形成引物二聚体或脱靶扩增。。因此，该算法能够为基于SYBR Green法的实时定量PCR检测实验设计出高效的引物序列。
为实现最佳的检测效果，RT2 lncRNA PCR Array应与RT2 First Strand Kit（用于cDNA合成）以及RT2 SYBR Green Mastermix（用于qPCR）联用。这些试剂专门针对RT2 lncRNA PCR Array配制，并经过了与RT2 lncRNA PCR Array的配合使用测试。RT2 First Strand Kit包含专有的基因组DNA去除步骤，可在反转录之前去除RNA样本中残留的基因组DNA污染，减少假阳性信号。此外，本品中还包含了一段人工合成的外源RNA对照模板，可供用户跟踪样品的反转录效率并监控RNase污染情况。不同定量PCR仪器专用的RT2 SYBR Green Mastermix都包含了SYBR Green与相应的参考染料，以匹配您实验检测所用的设备。RT2 SYBR Green Mastermix全面适用于QIAGEN、Applied Biosystems、Bio-Rad、Stratagene、Eppendorf、Roche及其他主要供应商的所有定量PCR仪器。
为实现最佳的检测效果，RT2 lncRNA PCR Array应与RT2 First Strand Kit（用于cDNA合成）以及RT2 SYBR Green Mastermix（用于qPCR）联用。这些试剂专门针对RT2 lncRNA PCR Array配制，并经过了与RT2 lncRNA PCR Array的配合使用测试。RT2 First Strand Kit包含专有的基因组DNA去除步骤，可在反转录之前去除RNA样本中残留的基因组DNA污染，减少假阳性信号。此外，本品中还包含了一段人工合成的外源RNA对照模板，可供用户跟踪样品的反转录效率并监控RNase污染情况。不同定量PCR仪器专用的RT2 SYBR Green Mastermix都包含了SYBR Green与相应的参考染料，以匹配您实验检测所用的设备。RT2 SYBR Green Mastermix全面适用于QIAGEN、Applied Biosystems、Bio-Rad、Stratagene、Eppendorf、Roche及其他主要供应商的所有定量PCR仪器。
Procedure
用户只需简单地将cDNA模板与适量的RT2 SYBR Green Mastermix即用型预混液混合，并等分到同一PCR板的每一反应孔中，再运行定量 PCR循环程序即可。RT2 lncRNA PCR Array可兼容QIAGEN、ABI、Bio-Rad、Eppendorf、Roche和Stratagene的全部仪器。
灵活的界面与操控
RT2 lncRNA PCR Array Modification提供96孔板、384孔板和100孔环状板规格，可用于检测与某种特定疾病状态或信号转导相关的84条lncRNA以及5个内参基因的表达情况。每一RT2 lncRNA PCR Array产品还提供以下功能：
灵活的界面与操控
RT2 lncRNA PCR Array Modification提供96孔板、384孔板和100孔环状板规格，可用于检测与某种特定疾病状态或信号转导相关的84条lncRNA以及5个内参基因的表达情况。每一RT2 lncRNA PCR Array产品还提供以下功能：
- 数据归一化
- 基因组DNA污染质控
- RNA样品质控
- PCR整体表现评估易用的数据分析
- 易用的数据分析
Applications
RT2 lncRNA PCR Array Modification非常适合于需要分析几种与整体信号转导或疾病相关的特定lncRNA的研究。
Supporting data and figures
Simple procedure.
The RT2 Profiler PCR Array system comprises a complete experimental workflow, from sample preparation through to data analysis and interpretation, starting with just 25 ng of RNA (or <1 ng of RNA with the RT2 PreAMP cDNA Synthesis Kit) and provides free data analysis software. Each cataloged RT2 Profiler PCR Array incorporates laboratory-verified assays for 84 pathway-focused genes, 5 housekeeping genes for normalization and controls that check for sample quality and reaction quality.
Resources
Safety Data Sheets (1)
Certificates of Analysis (1)