连接计算器
此工具确定了在适用于典型连接反应的范围内的各种插入片段:载体摩尔比率所需的插入片段质量。
见解
所需的 DNA 插入片段质量 [g] = 载体 DNA 质量 [g] x {DNA 插入片段长度 / 载体 DNA 长度} x 期望的插入片段与载体摩尔比率
提示：期望的比率为集合 {1, 2, 3, 5 ,7} 中的一个
插入片段长度应小于载体长度
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CAT NO./ID 203123
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50 µl AllTaq Polymerase (5 U/µl), 1.2 ml AllTaq PCR Buffer (5x), 55 µl dNTP Mix (10 mM each), 200 µl Template Tracer (25x), 50 µl Master Mix Tracer (125x), 2 ml Q-Solution (5x), 1.2 ml MgCl2 (25 mM), 1.9 ml RNase-Free Water
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Taq DNA Polymerase (250 U)
250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2
CAT NO./ID Y9140-LC-L
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CAT NO./ID 19092
Pathogen Lysis Tubes L
50 Pathogen Lysis Tubes with large beads, 1 vial of Reagent DX
CAT NO./ID 333777
QIAseq 96-Index L (384)
Box containing oligos for indexing up to 96 samples for QIAseq Targeted Panel sequencing on Ion Torrent platforms; enough to process a total of 384 samples
CAT NO./ID 333764
QIAseq 12-Index L (48)
Box containing oligos for indexing up to 12 samples for QIAseq Targeted Panel sequencing on Ion Torrent platforms; enough to process a total of 48 samples
CAT NO./ID 333491
QIAseq Targeted DNA Pro Index L (12)
Box containing oligos for indexing up to 12 samples for QIAseq Targeted DNA Pro Panel sequencing on Ion Torrent platforms
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CAT NO./ID 333127
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Kit containing sample indexes, enough for a total of 384 samples, for indexing up to 96 samples for targeted RNA sequencing on Illumina platforms (array format), and primers necessary for sequencing RNA libraries generated by the QIAseq targeted RNA panels on Illumina platforms
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artus Infl./H1 LC/RG RT-PCR Kit (24)
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artus Infl./H1 LC/RG RT-PCR Kit (96)
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T4 DNA Ligase (Rapid)
240,000 U of T4 DNA Ligase (600,000 U/mL) 2x Rapid Ligation Buffer and 10x T4 DNA Ligase Buffer
CAT NO./ID 19092
Pathogen Lysis Tubes L
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CAT NO./ID 334942
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QIAseq Targeted DNA Pro HC (12)
Kit containing all reagents (except indices) for targeted DNA sequencing; fixed panel for 12 samples
CAT NO./ID 333512
QIAseq Targeted DNA HC Panel (12)
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CAT NO./ID 333612
QIAseq Targeted RNAscan HC Panel (12)
Kit containing ALL reagents (except indexes) for targeted Fusion sequencing; fixed panel for 12 samples; more than 100 fusions
CAT NO./ID 333615
QIAseq Targeted RNAscan HC Panel (96)
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CAT NO./ID L6020L
T7 DNA Ligase (900,000 U)
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Klenow Fragment (2500 U)
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Ultra-Clean Production TaqNova DNA Polymerase.
5 U/µL concentration with reaction buffer of 10x TaqNova DNA-free Reaction Buffer, and 50 mM MgCl2Storage buffer: 20 mM Tris-HCl (pH 8.0 at 25°C); 100 mM KCl; 0.1 mM EDTA; 1 mM DTT; 0.5% (v/v) Nonident P40; 0.5% (v/v) Tween 20; 50% (v/v) glycerol
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