QIAamp DSP Circulating NA Kit

用于从人类样本中浓缩和纯化游离DNA、RNA和miRNA

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QIAamp DSP Circulating NA Kit

Cat. No. / ID: 61504

For 50 preps: includes QIAamp Mini Columns, Buffers, Carrier RNA, QIAGEN Proteinase K, and Tubes.

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QIAamp DSP Circulating NA Kit 旨在用于体外诊断用途。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

浓缩核酸；上样量达，洗脱体积小
高效回收片段化DNA、RNA和miRNA
无需有机抽提和乙醇沉淀
去除污染物和抑制剂

Product Details

QIAamp DSP Circulating NA Kit用于从血浆和血清中浓缩和纯化游离DNA和RNA。

Performance

The QIAamp DSP Circulating NA Kit易于使用，并符合GMP要求制造。此试剂盒可分离和纯化高品质核酸用于下游应用。在QIAvac 24 Plus上的真空处理使起始样本体积可达5 ml。灵活的洗脱体积在20 µl到150 µl之间，可对低浓度核酸进行浓缩。

Principle

QIAamp DSP Circulating NA Kit使用硅胶膜技术分离和纯化游离的、不含细胞的病毒核酸。

Procedure

QIAamp DSP Circulating NA Kit的操作流程包括4个步骤（裂解、结合、洗涤和洗脱），在真空底座上使用QIAamp Mini离心柱进行操作。核酸可被有效纯化和浓缩。完成裂解和上样步骤后，可在QIAcube全自动核酸纯化仪上自动纯化游离核酸。

Applications

QIAamp DSP Circulating NA Kit极大简化了从以下样本中分离游离的DNA和RNA：

血浆
血清
尿液

Supporting data and figures

QIAamp DSP Circulating NA Kit procedure.

Tube extenders for processing on the QIAvac 24 Plus.

Comparison of Classic and Breeze workflows.

Resources

QIAamp DSP Circulating NA Kit Performance Characteristics_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

For the Directive 98/79/EC (IVDD) compliant kit (kit version 1)

QIAamp DSP Circulating NA Kit Handbook_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

Download Safety Data Sheets for QIAGEN product components.

QIAamp DSP Circulating NA Kit Handbook_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

For the Directive 98/79/EC (IVDD) compliant kit (kit version 1)

QIAamp DSP Circulating NA Kit Performance Characteristics_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

Publications

Implementing prenatal diagnosis based on cell-free fetal DNA: accurate identification of factors affecting fetal DNA yield.

Barrett AN; Zimmermann BG; Wang D; Holloway A; Chitty LS;

PLoS One; 2011; 6 (10):e25202 2011 Oct 4 PMID:21998643

FAQ

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

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