Do you have a protocol for isolating RNA from yeast using RNeasy?

Yes, the RNeasy Mini Kit, RNeasy Midi Kit and RNeasy Maxi Kit can be used for this purpose. The RNeasy Mini Kit Handbook contains a standard and an abbreviated protocol using enzymatic lysis, and one protocol using mechanical disruption. The RNeasy Midi/Maxi Handbook contains a standard protocol for enzymatic lysis and one for mechanical disruption. The enzymatic protocols employ zymolase and lyticase, and the mechanical disruption protocols employ glass beads and a beadmill for cell wall disruption.


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