Why should I use the EpiTect Plus FFPE Bisulfite Kit instead of isolating FFPE DNA first and then doing the bisulfite conversion?

The EpiTect Plus FFPE Bisulfite Kit includes specialized buffers for efficient deparaffinization and lysis of FFPE tissue samples. After lysis DNA is directly bisulfite conversion without prior purification of DNA thus eliminating loss of DNA due to DNA isolation. In addition, the DNA Protect buffer included in the kit minimizes the DNA fragmentation due to bisulfite treatment.

In this combination, the yield of analyzable DNA can be increased 200 fold. A 25 fold due to direct isolation and 8-9 fold by DNA protection.


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