What are the maximum culture volumes to use with the QIAGEN Plasmid Midi or Maxi Kit?

Maximum recommended culture volumes for standard Luria Bertani (LB) medium*:

 

 

QIAGEN-tip 100

(QIAGEN Plasmid Midi Kit)

QIAGEN-tip 500

(QIAGEN Plasmid Maxi Kit)

High-copy plasmids

25 ml 100 ml

Low-copy plasmids

100 ml 500 ml


* For the QIAGEN-tip 100, the expected yields are 75–100 µg for high-copy plasmids and 20–100 µg for low-copy plasmids. For the QIAGEN-tip 500, the expected yields are 300–500 µg for high-copy plasmids and 100–500 µg for low-copy plasmids.

 

It is not recommended to use super rich growth media such as TB (terrific broth) or 2x YT for most commonly used high-copy plasmids. Although TB or 2x YT have the obvious advantage of producing more bacteria (2–5 times), this does not necessarily lead to greater yields or higher-quality DNA. Please visit the QIAGEN Plasmid Resource Center for further information on the growth of bacterial cultures.

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