Are there other options for paraffin removal?

Deparaffinization with the supplied Paraffin Removal Solution is recommended, however, other standard methods such as heptane and methanol or xylene can be used as well.
QIAGEN’s Deparaffinization Solution (DPS), catalog number 939018, can be used for removal of paraffin. For this adhere to the following procedure:
1. Place the FFPE sections in a 1.5 ml or 2 ml microcentrifuge tube (not supplied). Add 300 μl Deparaffinization Solution, vortex vigorously for 10 s, and centrifuge briefly to bring the sample to the bottom of the tube.
2. Incubate at 56°C for 3 min, then allow to cool to room temperature.
Note: If too little Deparaffinization Solution is used or if too much paraffin is carried over with the sample, Deparaffinization Solution may become waxy or solid after cooling. If this occurs, add additional Deparaffinization Solution and repeat the incubation at 56°C.
After this, proceed with step 7 of the EZ2 preparation in the Quick Start Protocol for the EZ2 RNA FFPE Kit.

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