Is it possible to test the efficiency of FastSelect rRNA removal by using a Bioanalyzer, etc.?

No, it is not possible to test the efficiency of the FastSelect reaction by running a portion of the eluate from the bead cleanup on a Bioanalyzer®, TapeStation®, Fragment Analyzer®, etc. FastSelect works by inhibiting reverse transcription of rRNA, which doesn’t occur until the first-strand synthesis reaction during library prep.


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