We strongly recommend to use the lysis buffer supplied with the PhosphoProtein Purification Kit
. If your cells cannot be lysed with this buffer, it is principally possible to use your own lysis buffer. In this case, it is crucial for purification that the lysed sample is dialyzed against the PhosphoProtein Lysis Buffer before loading the sample onto the column. Alternatively, you can also dilute your lysed sample 1:10 with the PhosphoProtein Lysis Buffer before loading the column.