Are there other options for paraffin removal?

QIAGEN’s Deparaffinization Solution (DPS) (cat. no. 939018) can be used for removal of paraffin. For this, adhere to the following procedure:

  1. Place the FFPE sections in a 1.5 ml or 2 ml microcentrifuge tube (not supplied). Add  300 μl DPS, vortex vigorously for 10 seconds, and centrifuge briefly to bring the sample to the bottom of the tube.
  2. Incubate at 56°C for 3 minutes, and then allow to cool to room temperature.
Note: If too little DPS is used or if too much paraffin is carried over with the sample, DPS may become waxy or solid after cooling. If this occurs, add additional DPS and repeat the incubation at 56°C. After this, proceed with step 3 of the Quick-Start Protocol for the EZ1&2 DNA FFPE and EZ1&2 DNA FFPE UNG Kits.

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