Can the REPLI-g FFPE procedure restore DNA derived from FFPE tissue samples in the original order?
No, the REPLI-g FFPE Kit cannot restore DNA derived from FFPE tissue samples in its original order. The sample fixation process results in fragmentation of the DNA, and the original order of fragments is lost.
In the REPLI-g FFPE procedure, fragments are eluted from the tissue section and are ligated randomly during the processing reaction of the protocol. However, this does not effect the detection of nucleic acid sequences, such as polymorhisms, in downstream applications.