What is the principle behind Effectene Transfection Reagent?

Effectene Reagent is a unique non-liposomal lipid formulation. Effectene Reagent is used in conjunction with an Enhancer and a DNA-condensation buffer (Buffer EC) to achieve high transfection efficiencies. In the first step of Effectene–DNA complex formation, the DNA is condensed by interaction with the Enhancer in a defined buffer system. Effectene Reagent is then added to the condensed DNA to produce Effectene–DNA complexes. The Effectene–DNA complexes are mixed with medium and directly added to the cells.

Effectene Reagent spontaneously forms micelle structures that show no size or batch variation, as found with preformulated liposome reagents. This unique feature ensures excellent reproducibility of transfection-complex formation. The process of condensing DNA molecules and then coating them with Effectene Reagent is a particularly effective way to transfer DNA into eukaryotic cells.

Broad cell line spectrum

Effectene Transfection Reagent has been used for transfection of a variety of different cell lines and primary cells, and yields significantly better transfection results than many widely used liposome-based transfection reagents. A searchable list of cell lines and primary cells successfully transfected using Effectene Reagent, as well as customer-developed protocols, is available at the Transfection Tools web site.


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