The composition of Buffer P1 is:
- 50 mM Tris·Cl, pH 8.0
- 10 mM EDTA
- 100 µg/ml RNase A
After RNase A addition, the buffer should be stored at 2–8°C.
Buffer P1 is the resuspension buffer used in a variety of QIAGEN kits for plasmid DNA purification. Details on buffer preparation and storage are presented in Appendix B of the QIAGEN Plasmid Purification Handbook.