The choice between one of two data analysis methods depends on the experimental setup and sample type.
1. ΔΔCt Method Recommended for experiments using: Small (four or less) number of fresh, frozen samples Large number of samples with similar DNA quality
2. Average Ct Method Recommended for experiments using: FPPE samples, large number of samples or without wild-type control samples
Data analysis can either be performed on the somatic mutation data analysis web portal or by downloading the Excel data analysis templates at http://www.sabiosciences.com/somaticmutationdataanalysis.php