What sequencing primers can I use with the pDrive cloning vector of the QIAGEN PCR Cloning Kit?

The pDrive cloning vector contains the standard sequencing annealing sites for M13 forward and reverse, as well as T7 and SP6 primers. Figure 1 in the QIAGEN PCR Cloning Kit Handbook shows the pDrive Cloning Vector Map. The positions of the T7 and SP6 promoter sites and the M13 forward and reverse sequencing primer binding sites are provided. You can find the primer sequences at the end of the Appendix in this handbook.



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