Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?
The initial centrifugations are performed at 6000 x g simply to reduce centrifuge noise. The final spin with the ethanolic wash buffer and the optional drying spin are both done at full speed to enhance ethanol drying from the spin column. Earlier centrifugation steps can also be performed at full speed, and this will not negatively affect DNA yield or quality.