Can the QIAprep Spin Miniprep Kit be used for isolating plasmid DNA from mammalian cells?

Yes, it is possible to isolate plasmid DNA from mammalian cells using the QIAprep Spin Miniprep kit . The article in QIAGEN News 1995 No. 2, page 11, Isolation of plasmid DNA from mammalian cells using QIAprep kit, describes a procedure that requires only 30 minutes compared to the time-consuming and labor-intensive Hirt method. 



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