Genotype of QIAGEN EZ Competent Cells supplied in the QIAGEN PCR Cloning plus Kit:
|Presence of the low-copy-number F plasmid [F'::Tn10(Tcr) proA+ B+ lacIq Z delta-M15]
|Transposon conferring tetracyclin resistance
|Overproduction of the lac operon repressor, which controls expression from Plac
|Expression of N-terminally deleted beta-galactosidase.This protein and LacZ alpha-peptide (encoded by the pDrive Cloning Vector) provide alpha-galactosidase activity. Cells transformed by pDrive Cloning Vector which does not contain a PCR product will express LacZ alpha-peptide, and will form blue colonies when grown in the presence of X-gal/IPTG. In contrast, cells transformed by pDrive Cloning Vector which does contain a PCR product will not express LacZ alpha-peptide, and will form white colonies.
|Abolished homologous recombination
|Abolished nonspecific endonuclease I activity, and hence improved DNA quality in plasmid preparations
|This mutation prevents restriction, but not protective methylation, of unmethylated DNA or DNA containing foreign methylation patterns in E. coli cells.
|Inability to utilize lactose
|Supressor of amber (UAG)mutation; required for growth of some phage vectors. Formerly called supE
|Requires thiamin (vitamin B1) for growth in minimal medium
|Mutation in DNA gyrase; resistance to naladixic acid
|Relaxed mutation, permits RNA synthesis in the absence of protein synthesis