Do you have a protocol for manual purification of 6xHis-tagged proteins expressed in E. coli using Ni-NTA Superflow?

Yes, please follow either of the QIAGEN Supplementary Protocols:

• 'Manual purification of 6xHis-tagged proteins from E. coli using the Ni-NTA Superflow 96 BioRobot Kit' (QE04)
• 'Manual purification of 6xHis-tagged proteins from E. coli using Ni-NTA Superflow Columns' (QE06)