3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol?
The lower limit of both protocols is identical but always uses the high concentration protocol if possible. The low concentration protocol allows for more DNA to be used by eliminating some of the protection buffer.
If the concentration of this DNA is sufficiently high (1ng or 100ng DNA) in 20µl, always use the high concentration protocol. If the DNA has a lower concentration, 40µl of DNA solution can be used at the expense of reducing the volume of Protect buffer (from 35 to 15µl). Because of this reduced protect buffer, the upper limit of input DNA for low concentrated samples is reduced from 2µg to 500ng to ensure consistently high DNA protection.This is also true for the new EpiTect Fast, where we have identical instructions in the handbook.