Can I do whole genome amplification from brain tissue using REPLI-g?
We have successfully amplified DNA from frozen brain tissue sections. A 2 mm section of frozen tissue should be used. Please follow the Supplementary protocol “Whole genome amplification from flash-frozen tissue sections using the REPLI-g® Midi Kit”
Alternatively, use a QIAGEN product for isolating genomic DNA from your specific starting material first, and then use the purified gDNA for whole genome amplification with the REPLI-g Mini/Midi, or take advantage of our REPLI-g WGA Services.