Can I use MspI or HpaII restriction enzymes from other suppliers?

The HpaII enzymes from Thermo Fisher Scientific ER0511, ER0512 and IVGN0936 have been extensively tested and show high compatibility with the QIAcuity Probe PCR chemistry. Use of MspI enzymes is not recommended. Compatibility of alternative enzymes must be individually tested and may need additional optimization.

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