How to quantitate DNA purified using the BioSprint 96?

Measure the absorbance of the eluate obtained on the BioSprint 96 instruments at 260 nm, 280 nm and 320 nm. Carryover of magnetic particles in the eluates may affect the A260 and A280 readings, but should not affect the performance of the DNA in downstream applications. Subtract the absorbance reading obtained at 320 nm from the readings obtained at 260 nm and 280 nm to correct for the presence of residual magnetic particles.


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