What is the composition of Buffer B2?

Buffer B2 is used in combination with Buffer B1, lysozyme or lysostaphin and Proteinase K for efficient lysis of bacteria prior to DNA purification using QIAGEN Genomic-tips. Genomic-tips are gravity-flow, anion-exchange columns. Please note this buffer is not recommended for any purification procedures using QIAGEN’s silica-membrane-based spin columns.

Buffer B2 (Bacterial Lysis Buffer 2) consists of 3 M guanidine hydrochloride, 20% Tween 20.

How to prepare Buffer B2: Dissolve 286.59 g guanidine hydrochloride in 700 ml distilled water. Add 200 ml 100% Tween 20. Adjust the volume to 1 liter with distilled water. pH does not need to be adjusted.


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