Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit?

When using the REPLI-g UltraFast Mini Kit with a reaction time reduced to 30 minutes, the DNA yield is extremely variable, ranging from zero to just a couple of micrograms. Therefore, we recommend a reaction time of at least 45 minutes. A reaction time of 60 minutes yields approximately 7–10 µg of DNA. However, to guarantee maximal yields of up to 10 µg, a reaction time of 90 minutes is recommended.



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