How can PCR cycling conditions be optimized for Phoenix Hot Start Taq?

General Taq protocols for PCR cycling (see recommended cycling conditions from Phoenix Hot Start Taq on Product Information Sheet) are a good starting point.  For determining the optimal temperature for the primer annealing step, vary the temperature by 2 degree increments starting at about 10 degrees below the Tm of the primer with the lower Tm up to about 5 degrees above Tm of primer with the higher Tm.

Phoenix Hot Start Taq is also compatible with Touchdown PCR cycling.  

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