The Normalization protocol is useful for routine, standardized analyses of reference samples, such as blood or buccal swabs, where sample-to-sample variability can result in widely varying DNA yields. The normalization protocol eliminates the need for yield quantification prior to analysis. It limits DNA yields uniformly to 150-250 ng.
However, please note that the Normalization protocol should not be used if the DNA amount in the sample is very limited. In this case, the Trace protocol on the EZ1 DNA Investigator Card should be used instead.